- GTX20292 - GeneTex COL1A1 antibody

Zhang M, Zhu P, Wang Y, Wu J, Yu Y, Wu X, Liu X, Gu Y
Journal of cellular and molecular medicine 2019 Feb;23(2):1001-1013

Schendel SA
Aesthetic surgery journal 2017 Oct 1;37(9):1062-1068

Chuang HM, Su HL, Li C, Lin SZ, Yen SY, Huang MH, Ho LI, Chiou TW, Harn HJ
Frontiers in pharmacology 2016;7:112

Chen MF, Huang SJ, Huang CC, Liu PS, Lin KI, Liu CW, Hsieh WC, Shiu LY, Chen CH
BMC cancer 2016 Jul 26;16:532

Choi JY, Shin MY, Suh SH, Park S
Biochemical and biophysical research communications 2015 Dec 25;468(4):883-8

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Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: Western blot analysis is shown using anti-Collagen I antibody to detect expression of collagen I in Wistar rat hepatic stellate cells (HSC) in control (GFP-transduced) (left lane) and PPARg-transduced cell lysates (right lane). Protein staining shown below each blot depicts equal protein loading. An equal amount of the whole cell protein (100 µg) was separated by SDS-PAGE and electroblotted to nitro-cellulose membranes. Proteins were detected by incubating the membrane with anti-Collagen I antibody at a concentration of 0.22 µg/10 ml in TBS (100 mM Tris-HCl, 0.15 M NaCl, pH 7.4) with 5% Non-fat milk. Detection occurred by incubation with a horseradish peroxidase-conjugated secondary antibody at 1 µg/10 ml.
Validation comment: WB

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: Western blot of Human Collagen Type I. Lane 1: Human Collagen Type 1. Lane 2: None. Load: 50 ng per lane. Primary antibody: Collagen Type I antibody (GTX20292) at 1:1,000 overnight at 4°C. Secondary antibody: DyLight 649 rabbit secondary antibody at 1:20,000 for 30 min at RT. Block for 30 min at RT. Predicted/Observed size: 139 & 130 kDa, 139 & 130 kDa for Collagen Type I. Other Band(s): Collagen Type I splice variants and isoforms
Validation comment: WB

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: Western blot analysis is shown using GeneTex Affinity Purified anti-Collagen I antibody (GTX20292) to detect expression of collagen I in Wistar rat hepatic stellate cells (HSC) in control (GFP-transduced) (left lane) and PPARg-transduced cell lysates (right lane). Protein staining shown below each blot depicts equal protein loading. An equal amount of the whole cell protein (100 µg) was separated by SDS-PAGE and electroblotted to nitro-cellulose membranes. Proteins were detected by incubating the membrane with anti-Collagen I antibody at a concentration of 0.22 µg/10 ml in TBS (100 mM Tris-HCl, 0.15 M NaCl, pH 7.4) with 5% Non-fat milk. Detection occurred by incubation with a horseradish peroxidase-conjugated secondary antibody at 1 µg/10 ml. Proteins were detected by a chemiluminescent method.
Validation comment: WB

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: Western blot of Human Collagen Type I. Load: 50 μg Human Collagen Type 1. Primary antibody: Collagen Type I antibody (GTX20292) at 1:1,000 overnight at 4°C. Secondary antibody: DyLight 649 rabbit secondary antibody at 1:20,000 for 30 min at RT. Block for 30 min at RT. Predicted/Observed size: 139 & 130 kDa.

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: Immunohistochemistry of Collagen I antibody (GTX20292) Tissue: human liung Fixation: formalin fixed paraffin embedded Antigen retrieval: user optimized Primary antibody: Collagen 1 1:400 Secondary antibody: Peroxidase goat anti-rabbit at 1:10,000 for 45 min at RT Localization: Strong staining was observed in the extracellular matrix of the lung. Epithelial cells were negative Staining: antibody as precipitated red signal with a hematoxylin purple nuclear counterstain

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: Immunohistochemistry of Collagen I antibody (GTX20292). Tissue: Normal Kidney. Fixation: formalin fixed paraffin embedded. Antigen retrieval: No antigen retrieval was performed. Primary antibody: Collagen I 1:100 4 hours at room temperature Secondary antibody: Peroxidase goat anti-rabbit at 1:10,000 for 45 min at RT. Localization: Distal tubules in normal kidney tissue. Note the absence of staining of glomeruli. Staining: antibody as precipitated red signal with a hematoxylin purple nuclear counterstain.

GTX20292