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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [1]
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Validation data
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- Product number
- PA5-35379 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Procollagen I N-Peptide Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- This antibody is predicted to react with most mammals, birds, amphibians and fish based on 100% sequence homology. This antibody contains enough material to conduct 10 mini-Western blots.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- ~0.15 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
Submitted references The mononuclear phagocyte system contributes to fibrosis in post-transplant obliterans bronchiolitis.
Di Campli MP, Azouz A, Assabban A, Scaillet J, Splittgerber M, Van Keymeulen A, Libert F, Remmelink M, Le Moine A, Lemaitre P, Goriely S
The European respiratory journal 2021 Mar;57(3)
The European respiratory journal 2021 Mar;57(3)
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Collagen I alpha 1 propeptide in rat lung lysate using a Collagen I alpha 1 propeptide polyclonal antibody (Product # PA5-35379). Results show a band at ~180kDa.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot of COL1A1 Propeptide in rat lung lysate showing specific immunolabeling of a band at ~180 kDa corresponding to COL1A1 Propeptide polyclonal antibody (Product # PA5-35379).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of COL1A1 Propeptide in fibrotic mouse lung tissue showing specific staining of COL1A1 Propeptide polyclonal antibody (Product # PA5-35379) using a dilution of 1:100 (red) that are still associated with the cells in which they were synthesized. The blue is staining DNA.
