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Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
- References [29]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [2]
- Immunohistochemistry [4]
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- Product number
- NB600-450 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB600-450, RRID:AB_10124390
- Product name
- Mouse Monoclonal Collagen I alpha 1 Antibody
- Antibody type
- Monoclonal
- Description
- Unpurified. This Collagen I alpha 1 antibody (COL-1) recognizes the native (helical) form of collagen type I using ELISA and dot-blot. It does not react with the thermally-denatured molecule, and shows no cross-reactivity with collagen types II, III, IV, V, VI, VII, IX, X and XI.
- Reactivity
- Human, Mouse, Rat, Bovine, Feline, Porcine, Rabbit
- Host
- Mouse
- Antigen sequence
The epitope recognized by the antib
ody may be sensitive to routine for
malin fixation and paraffin embeddi
ng.- Isotype
- IgG
- Vial size
- 0.1 ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Submitted references Size-Optimized Microspace Culture Facilitates Differentiation of Mouse Induced Pluripotent Stem Cells into Osteoid-Rich Bone Constructs.
Fast, robust and effective decellularization of whole human livers using mild detergents and pressure controlled perfusion.
Status of growth plates can be monitored by MRI.
In Vitro Fabrication of Hybrid Bone/Cartilage Complex Using Mouse Induced Pluripotent Stem Cells.
Radiation cystitis modeling: A comparative study of bladder fibrosis radio-sensitivity in C57BL/6, C3H, and BALB/c mice.
Rotator Cuff Repair With Autologous Tenocytes and Biodegradable Collagen Scaffold: A Histological and Biomechanical Study in Sheep.
Extracellular Matrix Features Discriminate Aggressive HER2-Positive Breast Cancer Patients Who Benefit from Trastuzumab Treatment.
Dynamic interactions between the extracellular matrix and estrogen activity in progression of ER+ breast cancer.
PCL-MECM-Based Hydrogel Hybrid Scaffolds and Meniscal Fibrochondrocytes Promote Whole Meniscus Regeneration in a Rabbit Meniscectomy Model.
Targeting the Epidermal Growth Factor Receptor Can Counteract the Inhibition of Natural Killer Cell Function Exerted by Colorectal Tumor-Associated Fibroblasts.
Development of a micro-tissue-mediated injectable bone tissue engineering strategy for large segmental bone defect treatment.
Role of A-Kinase Anchoring Protein Phosphorylation in Alcohol-Induced Liver Injury and Hepatic Stellate Cell Activation.
Advanced glycation end products as a source of artifacts in immunoenzymatic methods.
In Situ Cytokine Expression and Morphometric Evaluation of Total Collagen and Collagens Type I and Type III in Keloid Scars.
A Dual-Mode Bioreactor System for Tissue Engineered Vascular Models.
Expression of fibrosis-related molecules in the oral mucosa of six animal species: A reference for selecting animal models.
Fluorescence of Picrosirius Red Multiplexed With Immunohistochemistry for the Quantitative Assessment of Collagen in Tissue Sections.
Selective laser sintering scaffold with hierarchical architecture and gradient composition for osteochondral repair in rabbits.
Templated Assembly of Collagen Fibers Directs Cell Growth in 2D and 3D.
Effect of 1- and 6-Hour-Delayed Corneal Collagen Cross-Linking on Corneal Healing in a Rabbit Alkali-Burn Model: Clinical and Histological Observations.
Rotation-based technique for the rapid densification of tubular collagen gel scaffolds.
A novel open-porous magnesium scaffold with controllable microstructures and properties for bone regeneration.
Effect of a single injection of autologous conditioned serum (ACS) on tendon healing in equine naturally occurring tendinopathies.
Optimal amount of basic fibroblast growth factor in gelatin sponges incorporating β-tricalcium phosphate with chondrocytes.
Maspin influences response to doxorubicin by changing the tumor microenvironment organization.
Foxc2 over-expression in bone marrow mesenchymal stem cells stimulates osteogenic differentiation and inhibits adipogenic differentiation.
Affinity-selected heparan sulfate for bone repair.
Chondrogenesis of hMSC in affinity-bound TGF-beta scaffolds.
The influence of elastic components of the venous wall on the biomechanical properties of different veins used for arterial reconstruction.
Limraksasin P, Okawa H, Zhang M, Kondo T, Osathanon T, Pavasant P, Egusa H
Stem cells international 2020;2020:7082679
Stem cells international 2020;2020:7082679
Fast, robust and effective decellularization of whole human livers using mild detergents and pressure controlled perfusion.
Willemse J, Verstegen MMA, Vermeulen A, Schurink IJ, Roest HP, van der Laan LJW, de Jonge J
Materials science & engineering. C, Materials for biological applications 2020 Mar;108:110200
Materials science & engineering. C, Materials for biological applications 2020 Mar;108:110200
Status of growth plates can be monitored by MRI.
Wada H, Ikoma K, Oka Y, Nishida A, Onishi O, Kim WC, Tanida T, Yamada S, Matsuda KI, Tanaka M, Kubo T
Journal of magnetic resonance imaging : JMRI 2020 Jan;51(1):133-143
Journal of magnetic resonance imaging : JMRI 2020 Jan;51(1):133-143
In Vitro Fabrication of Hybrid Bone/Cartilage Complex Using Mouse Induced Pluripotent Stem Cells.
Limraksasin P, Kondo T, Zhang M, Okawa H, Osathanon T, Pavasant P, Egusa H
International journal of molecular sciences 2020 Jan 16;21(2)
International journal of molecular sciences 2020 Jan 16;21(2)
Radiation cystitis modeling: A comparative study of bladder fibrosis radio-sensitivity in C57BL/6, C3H, and BALB/c mice.
Zwaans BMM, Wegner KA, Bartolone SN, Vezina CM, Chancellor MB, Lamb LE
Physiological reports 2020 Feb;8(4):e14377
Physiological reports 2020 Feb;8(4):e14377
Rotator Cuff Repair With Autologous Tenocytes and Biodegradable Collagen Scaffold: A Histological and Biomechanical Study in Sheep.
Roßbach BP, Gülecyüz MF, Kempfert L, Pietschmann MF, Ullamann T, Ficklscherer A, Niethammer TR, Zhang A, Klar RM, Müller PE
The American journal of sports medicine 2020 Feb;48(2):450-459
The American journal of sports medicine 2020 Feb;48(2):450-459
Extracellular Matrix Features Discriminate Aggressive HER2-Positive Breast Cancer Patients Who Benefit from Trastuzumab Treatment.
Rybinska I, Sandri M, Bianchi F, Orlandi R, De Cecco L, Gasparini P, Campiglio M, Paolini B, Sfondrini L, Tagliabue E, Triulzi T
Cells 2020 Feb 13;9(2)
Cells 2020 Feb 13;9(2)
Dynamic interactions between the extracellular matrix and estrogen activity in progression of ER+ breast cancer.
Jallow F, O'Leary KA, Rugowski DE, Guerrero JF, Ponik SM, Schuler LA
Oncogene 2019 Oct;38(43):6913-6925
Oncogene 2019 Oct;38(43):6913-6925
PCL-MECM-Based Hydrogel Hybrid Scaffolds and Meniscal Fibrochondrocytes Promote Whole Meniscus Regeneration in a Rabbit Meniscectomy Model.
Chen M, Feng Z, Guo W, Yang D, Gao S, Li Y, Shen S, Yuan Z, Huang B, Zhang Y, Wang M, Li X, Hao L, Peng J, Liu S, Zhou Y, Guo Q
ACS applied materials & interfaces 2019 Nov 6;11(44):41626-41639
ACS applied materials & interfaces 2019 Nov 6;11(44):41626-41639
Targeting the Epidermal Growth Factor Receptor Can Counteract the Inhibition of Natural Killer Cell Function Exerted by Colorectal Tumor-Associated Fibroblasts.
Costa D, Venè R, Benelli R, Romairone E, Scabini S, Catellani S, Rebesco B, Mastracci L, Grillo F, Minghelli S, Loiacono F, Zocchi MR, Poggi A
Frontiers in immunology 2018;9:1150
Frontiers in immunology 2018;9:1150
Development of a micro-tissue-mediated injectable bone tissue engineering strategy for large segmental bone defect treatment.
Wu D, Wang Z, Wang J, Geng Y, Zhang Z, Li Y, Li Q, Zheng Z, Cao Y, Zhang ZY
Stem cell research & therapy 2018 Nov 28;9(1):331
Stem cell research & therapy 2018 Nov 28;9(1):331
Role of A-Kinase Anchoring Protein Phosphorylation in Alcohol-Induced Liver Injury and Hepatic Stellate Cell Activation.
Ramani K, Tomasi ML, Berlind J, Mavila N, Sun Z
The American journal of pathology 2018 Mar;188(3):640-655
The American journal of pathology 2018 Mar;188(3):640-655
Advanced glycation end products as a source of artifacts in immunoenzymatic methods.
Kuzan A, Chwiłkowska A, Maksymowicz K, Bronowicka-Szydełko A, Stach K, Pezowicz C, Gamian A
Glycoconjugate journal 2018 Feb;35(1):95-103
Glycoconjugate journal 2018 Feb;35(1):95-103
In Situ Cytokine Expression and Morphometric Evaluation of Total Collagen and Collagens Type I and Type III in Keloid Scars.
da Silva IR, Tiveron LCRDC, da Silva MV, Peixoto AB, Carneiro CAX, Dos Reis MA, Furtado PC, Rodrigues BR, Rodrigues V Jr, Rodrigues DBR
Mediators of inflammation 2017;2017:6573802
Mediators of inflammation 2017;2017:6573802
A Dual-Mode Bioreactor System for Tissue Engineered Vascular Models.
Bono N, Meghezi S, Soncini M, Piola M, Mantovani D, Fiore GB
Annals of biomedical engineering 2017 Jun;45(6):1496-1510
Annals of biomedical engineering 2017 Jun;45(6):1496-1510
Expression of fibrosis-related molecules in the oral mucosa of six animal species: A reference for selecting animal models.
Sa G, Xiong X, Ren J, Zhao Y, He S
European journal of pharmaceutical sciences : official journal of the European Federation for Pharmaceutical Sciences 2017 Jan 1;96:472-478
European journal of pharmaceutical sciences : official journal of the European Federation for Pharmaceutical Sciences 2017 Jan 1;96:472-478
Fluorescence of Picrosirius Red Multiplexed With Immunohistochemistry for the Quantitative Assessment of Collagen in Tissue Sections.
Wegner KA, Keikhosravi A, Eliceiri KW, Vezina CM
The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society 2017 Aug;65(8):479-490
The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society 2017 Aug;65(8):479-490
Selective laser sintering scaffold with hierarchical architecture and gradient composition for osteochondral repair in rabbits.
Du Y, Liu H, Yang Q, Wang S, Wang J, Ma J, Noh I, Mikos AG, Zhang S
Biomaterials 2017 Aug;137:37-48
Biomaterials 2017 Aug;137:37-48
Templated Assembly of Collagen Fibers Directs Cell Growth in 2D and 3D.
Liu GY, Agarwal R, Ko KR, Ruthven M, Sarhan HT, Frampton JP
Scientific reports 2017 Aug 29;7(1):9628
Scientific reports 2017 Aug 29;7(1):9628
Effect of 1- and 6-Hour-Delayed Corneal Collagen Cross-Linking on Corneal Healing in a Rabbit Alkali-Burn Model: Clinical and Histological Observations.
Karti O, Zengin MO, Cinar E, Tutuncu M, Karahan E, Celik A, Kucukerdonmez C
Cornea 2016 Dec;35(12):1644-1649
Cornea 2016 Dec;35(12):1644-1649
Rotation-based technique for the rapid densification of tubular collagen gel scaffolds.
Loy C, Lainé A, Mantovani D
Biotechnology journal 2016 Dec;11(12):1673-1679
Biotechnology journal 2016 Dec;11(12):1673-1679
A novel open-porous magnesium scaffold with controllable microstructures and properties for bone regeneration.
Cheng MQ, Wahafu T, Jiang GF, Liu W, Qiao YQ, Peng XC, Cheng T, Zhang XL, He G, Liu XY
Scientific reports 2016 Apr 13;6:24134
Scientific reports 2016 Apr 13;6:24134
Effect of a single injection of autologous conditioned serum (ACS) on tendon healing in equine naturally occurring tendinopathies.
Geburek F, Lietzau M, Beineke A, Rohn K, Stadler PM
Stem cell research & therapy 2015 Jun 26;6(1):126
Stem cell research & therapy 2015 Jun 26;6(1):126
Optimal amount of basic fibroblast growth factor in gelatin sponges incorporating β-tricalcium phosphate with chondrocytes.
Otani Y, Komura M, Komura H, Ishimaru T, Konishi K, Komuro H, Hoshi K, Takato T, Tabata Y, Iwanaka T
Tissue engineering. Part A 2015 Feb;21(3-4):627-36
Tissue engineering. Part A 2015 Feb;21(3-4):627-36
Maspin influences response to doxorubicin by changing the tumor microenvironment organization.
Triulzi T, Ratti M, Tortoreto M, Ghirelli C, Aiello P, Regondi V, Di Modica M, Cominetti D, Carcangiu ML, Moliterni A, Balsari A, Casalini P, Tagliabue E
International journal of cancer 2014 Jun 15;134(12):2789-97
International journal of cancer 2014 Jun 15;134(12):2789-97
Foxc2 over-expression in bone marrow mesenchymal stem cells stimulates osteogenic differentiation and inhibits adipogenic differentiation.
You W, Fan L, Duan D, Tian L, Dang X, Wang C, Wang K
Molecular and cellular biochemistry 2014 Jan;386(1-2):125-34
Molecular and cellular biochemistry 2014 Jan;386(1-2):125-34
Affinity-selected heparan sulfate for bone repair.
Murali S, Rai B, Dombrowski C, Lee JL, Lim ZX, Bramono DS, Ling L, Bell T, Hinkley S, Nathan SS, Hui JH, Wong HK, Nurcombe V, Cool SM
Biomaterials 2013 Jul;34(22):5594-605
Biomaterials 2013 Jul;34(22):5594-605
Chondrogenesis of hMSC in affinity-bound TGF-beta scaffolds.
Re'em T, Kaminer-Israeli Y, Ruvinov E, Cohen S
Biomaterials 2012 Jan;33(3):751-61
Biomaterials 2012 Jan;33(3):751-61
The influence of elastic components of the venous wall on the biomechanical properties of different veins used for arterial reconstruction.
Krasiński Z, Biskupski P, Dzieciuchowicz Ł, Kaczmarek E, Krasińska B, Staniszewski R, Pawlaczyk K, Stanisić M, Majewski P, Majewski W
European journal of vascular and endovascular surgery : the official journal of the European Society for Vascular Surgery 2010 Aug;40(2):224-9
European journal of vascular and endovascular surgery : the official journal of the European Society for Vascular Surgery 2010 Aug;40(2):224-9
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Collagen I alpha 1 Antibody (COL-1) [NB600-450] - Analysis of collagen 1. Lane 1: Recombinant human collagen 1 (3 ug). Lane 2: Pig skin whole cell lysate (20 ug) extracted with Laemmli buffer.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunocytochemistry/Immunofluorescence
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunocytochemistry/Immunofluorescence
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Frozen: Collagen I alpha 1 Antibody (COL-1) [NB600-450] - Staining of acetone-fixed, frozen sections of human tongue at a dilution of 1:2000.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Frozen: Collagen I alpha 1 Antibody (COL-1) [NB600-450] - Staining of acetone-fixed, frozen sections of pig tongue at a dilution of 1:4000
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry: Collagen I alpha 1 Antibody (COL-1) [NB600-450] - Analysis of an FFPE ligament section. Enzymatic antigen retrieval and blocking using 1% BSA for 30 min at 20C was performed. Sample was incubated for 1 hour with primary antibody at a dilution of 1:400 at 20C.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry: Collagen I alpha 1 Antibody (COL-1) [NB600-450] - Analysis of formalin fixed frozen porcine skin tissue sections. Blocking: 5% serum for 1 hr at 20C. Sample was incubated for 16 hours at 4C and used at a dilution of 1:500.
