Antibody data
- Antibody Data
- Antigen structure
- References [18]
- Comments [0]
- Validations
- Western blot [1]
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- Product number
- B00329 - Provider product page
- Provider
- Boster Biological Technology
- Product name
- Anti-COLLAGEN Type I COL1A1 Antibody Biotin Conjugated
- Antibody type
- Polyclonal
- Description
- Polyclonal antibody for COLLAGEN I/COL1A1 detection. Host: Rabbit.Size: 100μg. Tested applications: Flow Cytometry. Reactive species: Human. COLLAGEN I/COL1A1 information: Molecular Weight: 138941 MW; Subcellular Localization: Secreted, extracellular space, extracellular matrix ; Tissue Specificity: Forms the fibrils of tendon, ligaments and bones. In bones the fibrils are mineralized with calcium hydroxyapatite.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Conjugate
- Biotin
- Vial size
- 100μg
- Concentration
- 1.0 mg/mL by UV absorbance at 280 nm
- Storage
- At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquoted and stored frozen at -20°C for a longer time. Avoid repeated freezing and thawing.
Submitted references Impaired pulp healing associated with underlying disorders in the dental pulp of rats with type 2 diabetes.
N-methylpiperazine-diepoxyovatodiolide ameliorates peritoneal fibrosis via suppressing TGF-β/Smad and JAK/STAT signaling pathway.
Design, synthesis and anti-hepatic fibrosis activity of novel diphenyl vitamin D receptor agonists.
Epigallocatechin-3-Gallate Inhibits Atrial Fibrosis and Reduces the Occurrence and Maintenance of Atrial Fibrillation and its Possible Mechanisms.
Timosaponin BII inhibits TGF-β mediated epithelial-mesenchymal transition through Smad-dependent pathway during pulmonary fibrosis.
Patchouli alcohol against renal fibrosis of spontaneously hypertensive rats via Ras/Raf-1/ERK1/2 signalling pathway.
DNA from macrophages induces fibrosis and vasculopathy through POLR3A/STING/type I interferon axis in systemic sclerosis.
Connexin43 overexpression promotes bone regeneration by osteogenesis and angiogenesis in rat glucocorticoid-induced osteonecrosis of the femoral head.
Development of three-layer collagen scaffolds to spatially direct tissue-specific cell differentiation for enthesis repair.
Potential of an Aligned Porous Hydrogel Scaffold Combined with Periodontal Ligament Stem Cells or Gingival Mesenchymal Stem Cells to Promote Tissue Regeneration in Rat Periodontal Defects.
Inhibition of protein arginine methyltransferase 1 alleviates liver fibrosis by attenuating the activation of hepatic stellate cells in mice.
Bimodal fibrosis in a novel mouse model of bleomycin-induced usual interstitial pneumonia.
Tracing PRX1(+) cells during molar formation and periodontal ligament reconstruction.
Cathepsin B/NLRP3/GSDMD axis-mediated macrophage pyroptosis induces inflammation and fibrosis in systemic sclerosis.
Identification of Dental Stem Cells Similar to Skeletal Stem Cells.
Air pollution particles hijack peroxidasin to disrupt immunosurveillance and promote lung cancer.
Type I Collagen Suspension Induces Neocollagenesis and Myodifferentiation in Fibroblasts In Vitro.
MicroRNA-134 deactivates hepatic stellate cells by targeting TGF-β activated kinase 1-binding protein 1.
Tao S, Yang T, Zhou JN, Zhang Q
Journal of dental sciences 2024 Jan;19(1):310-320
Journal of dental sciences 2024 Jan;19(1):310-320
N-methylpiperazine-diepoxyovatodiolide ameliorates peritoneal fibrosis via suppressing TGF-β/Smad and JAK/STAT signaling pathway.
Mo M, Zeng Y, Zeng Y, Li S, He X, Chen X, Luo Q, Liu M, Luo C, Dou X, Peng F, Long H
Chemico-biological interactions 2023 Sep 1;382:110589
Chemico-biological interactions 2023 Sep 1;382:110589
Design, synthesis and anti-hepatic fibrosis activity of novel diphenyl vitamin D receptor agonists.
Xing K, Wu Y, Gao F, Dai Y, Guan C, Tong Y, Gao Y, Wang C, Zhang C
European journal of medicinal chemistry 2023 Oct 5;258:115596
European journal of medicinal chemistry 2023 Oct 5;258:115596
Epigallocatechin-3-Gallate Inhibits Atrial Fibrosis and Reduces the Occurrence and Maintenance of Atrial Fibrillation and its Possible Mechanisms.
Li T, Tong Q, Wang Z, Yang Z, Sun Y, Cai J, Xu Q, Lu Y, Liu X, Lin K, Qian Y
Cardiovascular drugs and therapy 2023 Mar 31;
Cardiovascular drugs and therapy 2023 Mar 31;
Timosaponin BII inhibits TGF-β mediated epithelial-mesenchymal transition through Smad-dependent pathway during pulmonary fibrosis.
Ding D, Shen X, Yu L, Zheng Y, Liu Y, Wang W, Liu L, Zhao Z, Nian S, Liu L
Phytotherapy research : PTR 2023 Jul;37(7):2787-2799
Phytotherapy research : PTR 2023 Jul;37(7):2787-2799
Patchouli alcohol against renal fibrosis of spontaneously hypertensive rats via Ras/Raf-1/ERK1/2 signalling pathway.
Li J, Hu G, Liu W, Cao X, Chen G, Peng F, Xiaofang X, Peng C
The Journal of pharmacy and pharmacology 2023 Jul 5;75(7):995-1010
The Journal of pharmacy and pharmacology 2023 Jul 5;75(7):995-1010
DNA from macrophages induces fibrosis and vasculopathy through POLR3A/STING/type I interferon axis in systemic sclerosis.
Liu C, Tang J, Luo W, Liu S, Sun X, Hong W, Zhou X, Lu J, Li M, Zhu L
Rheumatology (Oxford, England) 2023 Feb 1;62(2):934-945
Rheumatology (Oxford, England) 2023 Feb 1;62(2):934-945
Connexin43 overexpression promotes bone regeneration by osteogenesis and angiogenesis in rat glucocorticoid-induced osteonecrosis of the femoral head.
Zhao X, Chen C, Luo Y, Li D, Wang Q, Fang Y, Kang P
Developmental biology 2023 Apr;496:73-86
Developmental biology 2023 Apr;496:73-86
Development of three-layer collagen scaffolds to spatially direct tissue-specific cell differentiation for enthesis repair.
Pugliese E, Sallent I, Ribeiro S, Trotier A, Korntner SH, Bayon Y, Zeugolis DI
Materials today. Bio 2023 Apr;19:100584
Materials today. Bio 2023 Apr;19:100584
Potential of an Aligned Porous Hydrogel Scaffold Combined with Periodontal Ligament Stem Cells or Gingival Mesenchymal Stem Cells to Promote Tissue Regeneration in Rat Periodontal Defects.
Wang W, Wang A, Hu G, Bian M, Chen L, Zhao Q, Sun W, Wu Y
ACS biomaterials science & engineering 2023 Apr 10;9(4):1961-1975
ACS biomaterials science & engineering 2023 Apr 10;9(4):1961-1975
Inhibition of protein arginine methyltransferase 1 alleviates liver fibrosis by attenuating the activation of hepatic stellate cells in mice.
Yan FZ, Qian H, Liu F, Ding CH, Liu SQ, Xiao MC, Chen SJ, Zhang X, Luo C, Xie WF
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2022 Sep;36(9):e22489
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2022 Sep;36(9):e22489
Bimodal fibrosis in a novel mouse model of bleomycin-induced usual interstitial pneumonia.
Miura Y, Lam M, Bourke JE, Kanazawa S
Life science alliance 2022 Jan;5(1)
Life science alliance 2022 Jan;5(1)
Tracing PRX1(+) cells during molar formation and periodontal ligament reconstruction.
Gong X, Zhang H, Xu X, Ding Y, Yang X, Cheng Z, Tao D, Hu C, Xiang Y, Sun Y
International journal of oral science 2022 Jan 25;14(1):5
International journal of oral science 2022 Jan 25;14(1):5
Cathepsin B/NLRP3/GSDMD axis-mediated macrophage pyroptosis induces inflammation and fibrosis in systemic sclerosis.
Liu C, Tang J, Liu S, Shen C, Zhou X, Lu J, Li M, Zhu L
Journal of dermatological science 2022 Dec;108(3):127-137
Journal of dermatological science 2022 Dec;108(3):127-137
Identification of Dental Stem Cells Similar to Skeletal Stem Cells.
Liang JF, Wang J, Ji YT, Zhao Q, Han LT, Miron RJ, Zhang YF
Journal of dental research 2022 Aug;101(9):1092-1100
Journal of dental research 2022 Aug;101(9):1092-1100
Air pollution particles hijack peroxidasin to disrupt immunosurveillance and promote lung cancer.
Wang Z, Zhai Z, Chen C, Tian X, Xing Z, Xing P, Yang Y, Zhang J, Wang C, Dong L
eLife 2022 Apr 19;11
eLife 2022 Apr 19;11
Type I Collagen Suspension Induces Neocollagenesis and Myodifferentiation in Fibroblasts In Vitro.
Lombardi F, Palumbo P, Augello FR, Giusti I, Dolo V, Guerrini L, Cifone MG, Giuliani M, Cinque B
BioMed research international 2020;2020:6093974
BioMed research international 2020;2020:6093974
MicroRNA-134 deactivates hepatic stellate cells by targeting TGF-β activated kinase 1-binding protein 1.
Wang P, Lei S, Wang X, Xu W, Hu P, Chen F, Zhang X, Yin C, Xie W
Biochemistry and cell biology = Biochimie et biologie cellulaire 2019 Oct;97(5):505-512
Biochemistry and cell biology = Biochimie et biologie cellulaire 2019 Oct;97(5):505-512
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Supportive validation
- Submitted by
- Boster Biological Technology (provider)
- Main image
- Experimental details
- Western blot analysis of Collagen I expression in Wistar rat hepatic stellate cells (HSC) in control (GFP-transduced) (lane 1), PPARg-transduced cell lysates (lane 2). Load: 100 μg per lane. Collagen I at 138.9 kDa was detected using biotin conjugated rabbit anti-Collagen Type I Antigen Affinity purified polyclonal antibody (Catalog # B00329) at 0.2?? μg/10 ml. The blot was developed using chemiluminescence (ECL) method (Catalog # EK1002).
- Additional image