Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [2]
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Validation data
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- Product number
- LS-C745524 - Provider product page
- Provider
- LSBio
- Product name
- COL1A1 / Collagen I Alpha 1 Antibody (HRP) LS-C745524
- Antibody type
- Polyclonal
- Reactivity
- Human, Mouse, Rat, Bovine
- Host
- Rabbit
- Conjugate
- Horseradish Peroxidase
- Isotype
- IgG
- Storage
- Store vial at 4°C prior to restoration. For extended storage aliquot contents and freeze at -20°C or below. Avoid freeze-thaw cycles. Stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use.
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Supportive validation
- Submitted by
- LSBio (provider)
- Main image
- Experimental details
- Western Blot of rabbit anti-Collagen I antibody. Lane 1: Wistar rat hepatic stellate cells (HSC) in control (GFP-transduced). Lane 2: PPARg-transduced cell lysates. Load: 100 µg per lane. Protein staining shown below western blot depicts equal protein loading. Primary antibody: Anti-Collagen I antibody at 0.2–2 µg/10 ml for overnight at 4°C. Secondary antibody: horseradish peroxidase-conjugated rabbit secondary antibody at 1 µg/10 ml for overnight at 4°C. Block: TBS with 5% Non-fat milk. Predicted/Observed size: 138.9 kDa for Collagen I. Other band(s): none.
Supportive validation
- Submitted by
- LSBio (provider)
- Main image
- Experimental details
- Immunohistochemistry of rabbit Anti-Collagen Type I Antibody. Tissue: Human Skin at pH6. Fixation: formalin fixed paraffin embedded. Antigen retrieval: not required. Primary antibody: Collagen Type I antibody at 10 µg/mL for 1 h at RT. Secondary antibody: Peroxidase rabbit secondary antibody at 1:10,000 for 45 min at RT. Localization: Collagen Type I is secreted in the extracellular matrix. Staining: Collagen Type I as precipitated brown signal (A) with hematoxylin purple nuclear counterstain. With corresponding negative control (B).
- Submitted by
- LSBio (provider)
- Main image
- Experimental details
- Immunohistochemistry of rabbit Anti-Collagen Type I Antibody. Tissue: Human Skin at pH9. Fixation: formalin fixed paraffin embedded. Antigen retrieval: not required. Primary antibody: Collagen Type I antibody at 10 µg/mL for 1 h at RT. Secondary antibody: Peroxidase rabbit secondary antibody at 1:10,000 for 45 min at RT. Localization: Collagen Type I is secreted in the extracellular matrix. Staining: Collagen Type I as precipitated brown signal (A) with hematoxylin purple nuclear counterstain. With corresponding negative control (B).