LS-C745503

antibody from LSBio

Targeting: COL1A1 OI4

Western blot (Supportive data in Antibodypedia)

ELISA (Recommended by provider)

Immunoprecipitation (Recommended by provider)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

LS-C745503

Provider

LSBio

Product name

COL1A1 / Collagen I Alpha 1 Antibody (FITC) LS-C745503

Antibody type

Polyclonal

Reactivity

Human, Mouse, Rat, Bovine

Host

Rabbit

Conjugate

Green dye

Isotype

IgG

Storage

Store vial at 4°C prior to restoration. For extended storage aliquot contents and freeze at -20°C or below. Avoid freeze-thaw cycles. Stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use.

Western blot

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

Western Blot of rabbit anti-Collagen I antibody. Lane 1: Wistar rat hepatic stellate cells (HSC) in control (GFP-transduced). Lane 2: PPARg-transduced cell lysates. Load: 100 µg per lane. Protein staining shown below western blot depicts equal protein loading. Primary antibody: Anti-Collagen I antibody at 0.2–2 µg/10 ml for overnight at 4°C. Secondary antibody: horseradish peroxidase-conjugated rabbit secondary antibody at 1 µg/10 ml for overnight at 4°C. Block: TBS with 5% Non-fat milk. Predicted/Observed size: 138.9 kDa for Collagen I. Other band(s): none.

Immunohistochemistry

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

Immunohistochemistry of rabbit Anti-Collagen Type I Antibody. Tissue: Human Skin at pH6. Fixation: formalin fixed paraffin embedded. Antigen retrieval: not required. Primary antibody: Collagen Type I antibody at 10 µg/mL for 1 h at RT. Secondary antibody: Peroxidase rabbit secondary antibody at 1:10,000 for 45 min at RT. Localization: Collagen Type I is secreted in the extracellular matrix. Staining: Collagen Type I as precipitated brown signal (A) with hematoxylin purple nuclear counterstain. With corresponding negative control (B).

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

Immunohistochemistry of rabbit Anti-Collagen Type I Antibody. Tissue: Human Skin at pH9. Fixation: formalin fixed paraffin embedded. Antigen retrieval: not required. Primary antibody: Collagen Type I antibody at 10 µg/mL for 1 h at RT. Secondary antibody: Peroxidase rabbit secondary antibody at 1:10,000 for 45 min at RT. Localization: Collagen Type I is secreted in the extracellular matrix. Staining: Collagen Type I as precipitated brown signal (A) with hematoxylin purple nuclear counterstain. With corresponding negative control (B).