PA5-89281

antibody from Invitrogen Antibodies

Targeting: COL1A1 OI4

Western blot (Data presented on provider website)

ELISA (Recommended by provider)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-89281

Provider

Invitrogen Antibodies

Product name

COL1A1 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

Positive Samples: Mouse spleen, Mouse kidney, Rat liver, Rat lung; Cellular Location: extracellular matrix, extracellular space Immunogen sequence: PGKDGLNGLP GPIGPPGPRG RTGDAGPVGP PGPPGPPGPP GPPSAGFDFS FLPQPPQEKA HDGGRYYRAD DANVVRDRDL EVDTTLKSLS QQIENIRSPE G

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

1.73 mg/mL

Storage

-20°C, Avoid Freeze/Thaw Cycles

References

Submitted references

AKT3 deficiency in M2 macrophages impairs cutaneous wound healing by disrupting tissue remodeling.

Gu S, Dai H, Zhao X, Gui C, Gui J
Aging 2020 Apr 14;12(8):6928-6946

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescence analysis of COL1A1 in NIH/3T3 cells. Samples were incubated with COL1A1 Polyclonal antibody (Product # PA5-89281) using a dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescence analysis of COL1A1 in NIH/3T3 cells. Samples were incubated with COL1A1 Polyclonal antibody (Product # PA5-89281) using a dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescence analysis of COL1A1 in NIH/3T3 cells. Samples were incubated with COL1A1 Polyclonal antibody (Product # PA5-89281) using a dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of COL1A1 in paraffin-embedded human lung cancer. Samples were incubated with COL1A1 Polyclonal antibody (Product # PA5-89281) using a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of COL1A1 in paraffin-embedded mouse kidney. Samples were incubated with COL1A1 Polyclonal antibody (Product # PA5-89281) using a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of COL1A1 in paraffin-embedded rat ovary. Samples were incubated with COL1A1 Polyclonal antibody (Product # PA5-89281) using a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of COL1A1 in paraffin-embedded rat stomach. Samples were incubated with COL1A1 Polyclonal antibody (Product # PA5-89281) using a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of COL1A1 in paraffin-embedded human esophageal cancer. Samples were incubated with COL1A1 Polyclonal antibody (Product # PA5-89281) using a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of COL1A1 in paraffin-embedded human lung cancer. Samples were incubated with COL1A1 Polyclonal antibody (Product # PA5-89281) using a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of COL1A1 in paraffin-embedded mouse kidney. Samples were incubated with COL1A1 Polyclonal antibody (Product # PA5-89281) using a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of COL1A1 in paraffin-embedded rat ovary. Samples were incubated with COL1A1 Polyclonal antibody (Product # PA5-89281) using a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of COL1A1 in paraffin-embedded rat stomach. Samples were incubated with COL1A1 Polyclonal antibody (Product # PA5-89281) using a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of COL1A1 in paraffin-embedded human esophageal cancer. Samples were incubated with COL1A1 Polyclonal antibody (Product # PA5-89281) using a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.