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Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [24]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [1]
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- Product number
- NBP1-30054 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NBP1-30054, RRID:AB_1968486
- Product name
- Rabbit Polyclonal Collagen I alpha 1 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. This Collagen I alpha 1 antibody is specific for the ~ 140 kDa telopeptide portion of the collagen I alpha 1 polypeptide. The antibody works well for immunohistochemistry on paraformaldehyde-fixed sections with a simple antigen-retrieval protocol (incubate slides for 20 minutes at 90 degrees C in 10 mM sodium citrate (pH 6.0)/ 0.1 % Tween-20). Note that in paraffin sections of formaldehydefixed fibrotic mouse lung tissue, the antibody recognizes mature collagen I that has formed fibrils in the extracellular matrix.
- Reactivity
- Human, Mouse, Rat, Chicken/Avian, Sheep
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 ml
- Concentration
- 1.0 mg/ml
- Storage
- Store at -20C. Avoid freeze-thaw cycles.
Submitted references Syndecan-4 Protects the Heart From the Profibrotic Effects of Thrombin-Cleaved Osteopontin.
Hydrogen sulphide mitigates homocysteine-induced apoptosis and matrix remodelling in mesangial cells through Akt/FOXO1 signalling cascade.
PFKFB3 in Smooth Muscle Promotes Vascular Remodeling in Pulmonary Arterial Hypertension.
Reactive Oxygen Species-Dependent Calpain Activation Contributes to Airway and Pulmonary Vascular Remodeling in Chronic Obstructive Pulmonary Disease.
AIM2 gene silencing attenuates diabetic cardiomyopathy in type 2 diabetic rat model.
An optimised tissue disaggregation and data processing pipeline for characterising fibroblast phenotypes using single-cell RNA sequencing.
Dietary Sodium Restriction Reduces Arterial Stiffness, Vascular TGF-β-Dependent Fibrosis and Marinobufagenin in Young Normotensive Rats.
Cell-Specific Functions of ADAM17 Regulate the Progression of Thoracic Aortic Aneurysm.
Exendin-4 partly ameliorates - hyperglycemia-mediated tissue damage in lungs of streptozotocin-induced diabetic mice.
Inhibition of angiotensin II and calpain attenuates pleural fibrosis.
Dicer deficiency in proximal tubules exacerbates renal injury and tubulointerstitial fibrosis and upregulates Smad2/3.
BMP4 inhibits PDGF-induced proliferation and collagen synthesis via PKA-mediated inhibition of calpain-2 in pulmonary artery smooth muscle cells.
Mechanical regulation of cardiac fibroblast profibrotic phenotypes.
Hedgehog Signaling is Associated with Liver Response to Fractionated Irradiation in Mice.
Optimal Seeding Densities for In Vitro Chondrogenesis of Two- and Three-Dimensional-Isolated and -Expanded Bone Marrow-Derived Mesenchymal Stromal Stem Cells Within a Porous Collagen Scaffold.
Persistent activation of autophagy in kidney tubular cells promotes renal interstitial fibrosis during unilateral ureteral obstruction.
RNA-binding Protein Musashi Homologue 1 Regulates Kidney Fibrosis by Translational Inhibition of p21 and Numb mRNA.
Relationship of advanced glycation end products and their receptor to pelvic organ prolapse.
The copper chelator tetrathiomolybdate regressed bleomycin-induced pulmonary fibrosis in mice, by reducing lysyl oxidase expressions.
Decellularized ovine arteries as small-diameter vascular grafts.
A role for hedgehog signaling in the differentiation of the insertion site of the patellar tendon in the mouse.
Diet-induced mouse model of fatty liver disease and nonalcoholic steatohepatitis reflecting clinical disease progression and methods of assessment.
Spatial and temporal expression of molecular markers and cell signals during normal development of the mouse patellar tendon.
Glucagon-like peptide-1 receptor agonism improves metabolic, biochemical, and histopathological indices of nonalcoholic steatohepatitis in mice.
Herum KM, Romaine A, Wang A, Melleby AO, Strand ME, Pacheco J, Braathen B, Dunér P, Tønnessen T, Lunde IG, Sjaastad I, Brakebusch C, McCulloch AD, Gomez MF, Carlson CR, Christensen G
Journal of the American Heart Association 2020 Feb 4;9(3):e013518
Journal of the American Heart Association 2020 Feb 4;9(3):e013518
Hydrogen sulphide mitigates homocysteine-induced apoptosis and matrix remodelling in mesangial cells through Akt/FOXO1 signalling cascade.
Majumder S, Ren L, Pushpakumar S, Sen U
Cellular signalling 2019 Sep;61:66-77
Cellular signalling 2019 Sep;61:66-77
PFKFB3 in Smooth Muscle Promotes Vascular Remodeling in Pulmonary Arterial Hypertension.
Kovacs L, Cao Y, Han W, Meadows L, Kovacs-Kasa A, Kondrikov D, Verin AD, Barman SA, Dong Z, Huo Y, Su Y
American journal of respiratory and critical care medicine 2019 Sep 1;200(5):617-627
American journal of respiratory and critical care medicine 2019 Sep 1;200(5):617-627
Reactive Oxygen Species-Dependent Calpain Activation Contributes to Airway and Pulmonary Vascular Remodeling in Chronic Obstructive Pulmonary Disease.
Zhu J, Kovacs L, Han W, Liu G, Huo Y, Lucas R, Fulton D, Greer PA, Su Y
Antioxidants & redox signaling 2019 Oct 20;31(12):804-818
Antioxidants & redox signaling 2019 Oct 20;31(12):804-818
AIM2 gene silencing attenuates diabetic cardiomyopathy in type 2 diabetic rat model.
Wang X, Pan J, Liu H, Zhang M, Liu D, Lu L, Tian J, Liu M, Jin T, An F
Life sciences 2019 Mar 15;221:249-258
Life sciences 2019 Mar 15;221:249-258
An optimised tissue disaggregation and data processing pipeline for characterising fibroblast phenotypes using single-cell RNA sequencing.
Waise S, Parker R, Rose-Zerilli MJJ, Layfield DM, Wood O, West J, Ottensmeier CH, Thomas GJ, Hanley CJ
Scientific reports 2019 Jul 3;9(1):9580
Scientific reports 2019 Jul 3;9(1):9580
Dietary Sodium Restriction Reduces Arterial Stiffness, Vascular TGF-β-Dependent Fibrosis and Marinobufagenin in Young Normotensive Rats.
Grigorova YN, Wei W, Petrashevskaya N, Zernetkina V, Juhasz O, Fenner R, Gilbert C, Lakatta EG, Shapiro JI, Bagrov AY, Fedorova OV
International journal of molecular sciences 2018 Oct 15;19(10)
International journal of molecular sciences 2018 Oct 15;19(10)
Cell-Specific Functions of ADAM17 Regulate the Progression of Thoracic Aortic Aneurysm.
Shen M, Hu M, Fedak PWM, Oudit GY, Kassiri Z
Circulation research 2018 Jul 20;123(3):372-388
Circulation research 2018 Jul 20;123(3):372-388
Exendin-4 partly ameliorates - hyperglycemia-mediated tissue damage in lungs of streptozotocin-induced diabetic mice.
Oztay F, Sancar-Bas S, Gezginci-Oktayoglu S, Ercin M, Bolkent S
Peptides 2018 Jan;99:99-107
Peptides 2018 Jan;99:99-107
Inhibition of angiotensin II and calpain attenuates pleural fibrosis.
Song LJ, Xiang F, Ye H, Huang H, Yang J, Yu F, Xiong L, Xu JJ, Greer PA, Shi HZ, Xin JB, Su Y, Ma WL
Pulmonary pharmacology & therapeutics 2018 Feb;48:46-52
Pulmonary pharmacology & therapeutics 2018 Feb;48:46-52
Dicer deficiency in proximal tubules exacerbates renal injury and tubulointerstitial fibrosis and upregulates Smad2/3.
Ma Z, Wei Q, Zhang M, Chen JK, Dong Z
American journal of physiology. Renal physiology 2018 Dec 1;315(6):F1822-F1832
American journal of physiology. Renal physiology 2018 Dec 1;315(6):F1822-F1832
BMP4 inhibits PDGF-induced proliferation and collagen synthesis via PKA-mediated inhibition of calpain-2 in pulmonary artery smooth muscle cells.
Cai P, Kovacs L, Dong S, Wu G, Su Y
American journal of physiology. Lung cellular and molecular physiology 2017 May 1;312(5):L638-L648
American journal of physiology. Lung cellular and molecular physiology 2017 May 1;312(5):L638-L648
Mechanical regulation of cardiac fibroblast profibrotic phenotypes.
Herum KM, Choppe J, Kumar A, Engler AJ, McCulloch AD
Molecular biology of the cell 2017 Jul 7;28(14):1871-1882
Molecular biology of the cell 2017 Jul 7;28(14):1871-1882
Hedgehog Signaling is Associated with Liver Response to Fractionated Irradiation in Mice.
Kim J, Wang S, Hyun J, Guy CD, Jung Y
Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology 2016;40(1-2):263-276
Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology 2016;40(1-2):263-276
Optimal Seeding Densities for In Vitro Chondrogenesis of Two- and Three-Dimensional-Isolated and -Expanded Bone Marrow-Derived Mesenchymal Stromal Stem Cells Within a Porous Collagen Scaffold.
Bornes TD, Jomha NM, Mulet-Sierra A, Adesida AB
Tissue engineering. Part C, Methods 2016 Mar;22(3):208-20
Tissue engineering. Part C, Methods 2016 Mar;22(3):208-20
Persistent activation of autophagy in kidney tubular cells promotes renal interstitial fibrosis during unilateral ureteral obstruction.
Livingston MJ, Ding HF, Huang S, Hill JA, Yin XM, Dong Z
Autophagy 2016 Jun 2;12(6):976-98
Autophagy 2016 Jun 2;12(6):976-98
RNA-binding Protein Musashi Homologue 1 Regulates Kidney Fibrosis by Translational Inhibition of p21 and Numb mRNA.
Jadhav S, Ajay AK, Trivedi P, Seematti J, Pellegrini K, Craciun F, Vaidya VS
The Journal of biological chemistry 2016 Jul 1;291(27):14085-14094
The Journal of biological chemistry 2016 Jul 1;291(27):14085-14094
Relationship of advanced glycation end products and their receptor to pelvic organ prolapse.
Chen Y, Huang J, Hu C, Hua K
International journal of clinical and experimental pathology 2015;8(3):2288-99
International journal of clinical and experimental pathology 2015;8(3):2288-99
The copper chelator tetrathiomolybdate regressed bleomycin-induced pulmonary fibrosis in mice, by reducing lysyl oxidase expressions.
Ovet H, Oztay F
Biological trace element research 2014 Dec;162(1-3):189-99
Biological trace element research 2014 Dec;162(1-3):189-99
Decellularized ovine arteries as small-diameter vascular grafts.
Mancuso L, Gualerzi A, Boschetti F, Loy F, Cao G
Biomedical materials (Bristol, England) 2014 Aug;9(4):045011
Biomedical materials (Bristol, England) 2014 Aug;9(4):045011
A role for hedgehog signaling in the differentiation of the insertion site of the patellar tendon in the mouse.
Liu CF, Breidenbach A, Aschbacher-Smith L, Butler D, Wylie C
PloS one 2013;8(6):e65411
PloS one 2013;8(6):e65411
Diet-induced mouse model of fatty liver disease and nonalcoholic steatohepatitis reflecting clinical disease progression and methods of assessment.
Clapper JR, Hendricks MD, Gu G, Wittmer C, Dolman CS, Herich J, Athanacio J, Villescaz C, Ghosh SS, Heilig JS, Lowe C, Roth JD
American journal of physiology. Gastrointestinal and liver physiology 2013 Oct 1;305(7):G483-95
American journal of physiology. Gastrointestinal and liver physiology 2013 Oct 1;305(7):G483-95
Spatial and temporal expression of molecular markers and cell signals during normal development of the mouse patellar tendon.
Liu CF, Aschbacher-Smith L, Barthelery NJ, Dyment N, Butler D, Wylie C
Tissue engineering. Part A 2012 Mar;18(5-6):598-608
Tissue engineering. Part A 2012 Mar;18(5-6):598-608
Glucagon-like peptide-1 receptor agonism improves metabolic, biochemical, and histopathological indices of nonalcoholic steatohepatitis in mice.
Trevaskis JL, Griffin PS, Wittmer C, Neuschwander-Tetri BA, Brunt EM, Dolman CS, Erickson MR, Napora J, Parkes DG, Roth JD
American journal of physiology. Gastrointestinal and liver physiology 2012 Apr 15;302(8):G762-72
American journal of physiology. Gastrointestinal and liver physiology 2012 Apr 15;302(8):G762-72
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Simple Western: Collagen I alpha 1 Antibody [NBP1-30054] - Simple Western lane view shows a specific band for Collagen I Alpha 1 in 0.5 mg/ml of Human Lung (left) and Human Kidney (right) lysate. This experiment was performed under reducing conditions using the 66-440 kDa separation system. * Non-specific interaction with the 230 kDa Simple Western standard may be seen with this antibody
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Collagen I alpha 1 Antibody [NBP1-30054] - Collagen I Antibody [NBP1-30054] - Rat lung lysate showing specific immunolabeling of the collagen protein with an observed molecular weight ~140.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry: Collagen I alpha 1 Antibody [NBP1-30054] - Collagen I Antibody [NBP1-30054] - Formaldehyde-fixed fibrotic mouse lung tissue. The antibody recognizes mature collagen I (red) that has formed fibrils in the extracellular matrix.
