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LearnPA5-41723
antibody from Invitrogen Antibodies
Targeting: SF3B1
Hsh155, Prp10, PRPF10, SAP155, SF3b155
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [2]
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- Product number
- PA5-41723 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- SF3B1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- Peptide sequence: LLNDIPQSTE QYDPFAEHRP PKIADREDEY KKHRRTMIIS PERLDPFADG
- Concentration
- 1 mg/mL
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of human Jurkat cell lysate using an anti-SF3B1 polyclonal antibody (Product # PA5-41723).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis was performed on modified whole cell extracts (1% SDS) (30 µg lysate) of HeLa (Lane 1), PANC-1 (Lane 2), A-431 (Lane 3), A549 (Lane 4), U-2 OS (Lane 5), HCT 116 (Lane 6) and MCF7 (Lane 7). The blot was probed with SF3B1 Polyclonal Antibody (Product # PA5-41723, 1µg/ml) and detected by chemiluminescence using Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.25 µg/ml, 1:4000 dilution). A 146 kDa band corresponding to SF3B1 was observed across the cell lines tested.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry (paraffin-embedded) analysis of human cardiac tissue using an anti-SF3B1 polyclonal antibody (Product # PA5-41723).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry (paraffin-embedded) analysis of human liver tissue using an anti-SF3B1 polyclonal antibody (Product # PA5-41723).
