PA5-47949

antibody from Invitrogen Antibodies

Targeting: BMPR2 BMPR-II, BMPR3, BRK-3, PPH1, T-ALK

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-47949

Provider

Invitrogen Antibodies

Product name

BMPR2 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant full-length protein

Description

In direct ELISAs, less than 1% cross-reactivity with recombinant human (rh) BMPR-IA, recombinant mouse (rm) BMPR-IA, rhBMPR-IB, and rmBMPR-IB is observed.

Concentration

0.2 mg/mL

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Knockdown of BMPR2 was achieved by transfecting A-431 cells with BMPR2 specific siRNAs (Silencer® select Product # s2044). Western blot analysis (Fig. a) was performed using whole cell extracts from the BMPR2 knockdown cells (Lane 3), non-specific scrambled siRNA transfected cells (Lane 2) and untransfected cells (Lane 1). The blot was probed with Anti-BMPR2 Polyclonal Antibody (Product # PA5-47949, 0.1µg/ml) and Goat anti-Rabbit IgG (H+L), Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Densitometric analysis of this Western Blot is shown in histogram (Fig. b). Decrease in signal upon siRNA mediated knock down confirms that antibody is specific to BMPR2.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot was performed using Anti-BMPR2 Polyclonal Antibody (Product # PA5-47949) and a 115 kDa band corresponding to BMPR2 was observed across the cell lines and tissues tested. Whole cell extracts (30 µg lysate) of Raji (Lane 1), A-431 (Lane 2), Daudi (Lane 3), HL-60 (Lane 4) and tissue extract (30 µg lysate) of Mouse Brain (Lane 5) were electrophoresed using Novex® NuPAGE® 4-12 % Bis-Tris gel (Product # NP0322BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:2000 dilution) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L), Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of BMPR2 in formalin fixed paraffin-embedded sections of human prostate. Samples were incubated in BMPR2 polyclonal antibody (Product # PA5-47949) using a dilution of 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown) and counterstained with hematoxylin (blue). Specific labeling was localized to the plasma membrane of epithelial cells.

Flow cytometry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Flow cytometry of BMPR2 in PC‚3 human prostate cancer cell line. Samples were incubated in BMPR2 polyclonal antibody (Product # PA5-47949) or control antibody followed by Allophycocyanin-conjugated Anti-Goat IgG Secondary Antibody.