PA5-21437

antibody from Invitrogen Antibodies

Targeting: BMPR2 BMPR-II, BMPR3, BRK-3, PPH1, T-ALK

Antibody data

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Validation data

BMPR2 protein structure - PA5-21437 shown in red.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of BMPR2 using 30 µg of A) H1299 and B) Raji lysate. Samples were loaded onto a 7.5% SDS-PAGE gel and probed with a BMPR2 polyclonal antibody (Product # PA5-21437) at a dilution of 1:1000.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of BMPR2 using 30 µg of NIH-3T3 lysate. Samples were loaded onto a 7.5% SDS-PAGE gel and probed with a BMPR2 polyclonal antibody (Product # PA5-21437) at a dilution of 1:1000.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western Blot analysis of BMPR2 was performed by separating 30 µg of various whole cell extracts by 5% SDS-PAGE. Proteins were transferred to a membrane and probed with a BMPR2 Polyclonal Antibody (Product # PA5-21437) at a dilution of 1:500 and a HRP-conjugated anti-rabbit IgG secondary antibody.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot was performed using Anti-BMPR2 Polyclonal Antibody (Product # PA5-21437) and a 115 kDa band corresponding to BMPR2 was observed across the cell lines and tissues tested. Whole cell extracts (30 µg lysate) of HUV-EC-C (Lane 1), U-2 OS (Lane 2), tissue extracts (30 µg lysate) of Mouse Brain (Lane 3) and Rat Brain (Lane 4) were electrophoresed using Novex® NuPAGE® 4-12 % Bis-Tris gel (Product # NP0322BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:500 dilution) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L), Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot was performed using Anti-BMPR2 Polyclonal Antibody (Product # PA5-21437) and a 115 kDa band corresponding to BMPR2 was observed in Mouse Brain when compared to Mouse Esophagus which is reported to be negative model for BMPR2 expression. Tissue extracts (30 µg lysate) of Mouse Brain (Lane 1) and Mouse Esophagus (Lane 2) were electrophoresed using Novex® NuPAGE® 4-12 % Bis-Tris gel (Product # NP0322BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:500 dilution) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L), Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).