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Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Flow cytometry [1]
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- Product number
- NBP2-42630 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Mouse Monoclonal CTLA-4 Antibody
- Antibody type
- Monoclonal
- Description
- Protein A purified.
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 100 ul
- Concentration
- 1 mg/ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: CTLA-4 Antibody (4C8) [NBP2-42630] - The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human CTLA4 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: Mouse liver tissue lysate Lane 2.: TF-1 cell lysate Lane 3.: HepG2 cell lysate Lane 4.: WiDr cell lysate
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: CTLA-4 Antibody (4C8) [NBP2-42630] - Analysis in Jurkat cells. The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: CTLA-4 Antibody (4C8) [NBP2-42630] - Analysis of CTLA4 in HeLa cells. The cell was stained with at 2-5ug for 1x106cells (red). A Goat anti mouse IgG (Alexa fluor 488) was used as the secondary antibody. Mouse monoclonal IgG was used as the isotype control (dark gray), cells without incubation with primary and secondary antibody was used as the negative control (light gray).
