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antibody from Invitrogen Antibodies
Targeting: CTLA4
CD, CD152, CELIAC3, CTLA-4, GSE, IDDM12
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Immunocytochemistry [2]
- Flow cytometry [1]
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- Product number
- PA5-47547 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- CTLA-4 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- In Western blots, approximately 25% cross-reactivity with recombinant mouse CTLA-4 is observed.
- Concentration
- 0.2 mg/mL
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemical analysis of CTLA-4 was detected in immersion fixed human peripheral blood mononuclear cells treated with treated with PMA and calcium ionomycin using Goat Anti-human CTLA-4 Antigen Affinity-purified Polyclonal Antibody (Product # PA5-47547) at 15 µg/mL for 3 hours at room temperature. Cells were stained using the 557-conjugated Anti-Goat IgG Secondary Antibody (re and counterstained with DAPI (blue). Specific staining was localized to cell surfaces.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemical analysis of CTLA-4 was detected in immersion fixed human peripheral blood mononuclear cells treated with treated with PMA and calcium ionomycin using Goat Anti-human CTLA-4 Antigen Affinity-purified Polyclonal Antibody (Product # PA5-47547) at 15 µg/mL for 3 hours at room temperature. Cells were stained using the 557-conjugated Anti-Goat IgG Secondary Antibody (re and counterstained with DAPI (blue). Specific staining was localized to cell surfaces.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometric analysis of NS0 mouse myeloma cell line co-transfected with human CTLA-4 and eGFP was stained with either (A) Goat Anti-human CTLA-4 Antigen Affinity-purified Polyclonal Antibody (Product # PA5-47547) or (B) Normal Goat IgG Control followed by Allophycocyanin-conjugated Anti-Goat IgG Secondary Antibody.
