702534

antibody from Invitrogen Antibodies

Targeting: CTLA4 CD, CD152, CELIAC3, CTLA-4, GSE, IDDM12

Immunocytochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

702534

Provider

Invitrogen Antibodies

Product name

CTLA-4 Recombinant Rabbit Monoclonal Antibody (11H7L17)

Antibody type

Monoclonal

Antigen

Other

Description

This antibody is predicted to react with Monkey, Cat, Pig

Antibody clone number

11H7L17

Concentration

0.5 mg/mL

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

For immunofluorescence analysis, Jurkat cells were fixed and permeabilized for detection of endogenous CTLA4 using Anti-CTLA4 Recombinant Rabbit Monoclonal Antibody (Product # 702534, 2 µg/mL) and labeled with Goat anti-Rabbit IgG (H+L) Superclonal Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034, 1:2000). Panel a) shows representative cells that were stained for detection and localization of CTLA4 protein (green), Panel b) is stained for nuclei (blue) using SlowFade® Gold Antifade Mountant with DAPI (Product # S36938). Panel c) represents cytoskeletal F-actin staining using Rhodamine Phalloidin (Product # R415, 1:300). Panel d) is a composite image of Panels a, b and c clearly demonstrating staining in the membrane (green) in cells treated with PMA (5 ng/mL, 48hrs). Panel e) demonstrates lower signal intensity in untreated cells. Panel f) represents control cells with no primary antibody to assess background. The images were captured at 60X magnification.