711564

antibody from Invitrogen Antibodies

Targeting: CTLA4 CD, CD152, CELIAC3, CTLA-4, GSE, IDDM12

Immunocytochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

711564

Provider

Invitrogen Antibodies

Product name

CTLA-4 Recombinant Polyclonal Antibody (11HCLC)

Antibody type

Polyclonal

Antigen

Other

Description

This antibody is predicted to react with Monkey, Pig and Cat.

Antibody clone number

11HCLC

Concentration

0.5 mg/mL

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

For immunofluorescence analysis, Jurkat cells were fixed and permeabilized for detection of endogenous CTLA4 using CTLA4 Recombinant Rabbit Polyclonal Antibody (Product # 711564, 2 µg/mL) and labeled with Goat anti-Rabbit IgG (H+L) Superclonal Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034, 1:2000). Panel a) shows representative cells that were stained for detection and localization of CTLA4 protein (green), Panel b) is stained for nuclei (blue) using SlowFade® Gold Antifade Mountant with DAPI (Product # S36938). Panel c) represents cytoskeletal F-actin staining using Alexa Fluor® 555 Rhodamine Phalloidin (Product # R415, 1:300). Panel d) is a composite image of Panels a, b and c clearly demonstrating localization of CTLA4 in the membrane. Panel e) represents control cells with no primary antibody to assess background. The images were captured at 60X magnification.