Antibody data
- Antibody Data
- Antigen structure
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- Validations
- ELISA [6]
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Validation data
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- Product number
- LS-C742108 - Provider product page
- Provider
- LSBio
- Product name
- CTLA4 / CD152 Antibody (clone MCT.E9) LS-C742108
- Antibody type
- Monoclonal
- Description
- Protein A affinity chromatography
- Reactivity
- Mouse
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- MCT.E9
- Storage
- Long term: store at -20°C for up to 2 years. Reconstituted: store at 2°C to 8°C for up to 3 weeks or at -20°C for up to 1 year. Avoid freeze/thaw cycles.
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Supportive validation
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- ELISA blocking of mouse CTLA4 antibody MCT.E9 against mouse CD86 recombinant protein binding with Mouse CTLA4 recombinant protein. Coating antigen: CD86, 0.5 µg/ml. Mouse CTLA4 final concentration: 2 µg /ml CTLA4 antibody dilution start from 50 µg/ml, IC50=0.1494 µg/ml
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- ELISA blocking of mouse CTLA4 antibody MCT.E9 against mouse CD80 recombinant protein binding with Mouse CTLA4 recombinant protein. Coating antigen: CD80, 0.5 µg/ml. Mouse CTLA4 final concentration: 0.5 µg /ml CTLA4 antibody dilution start from 50 µg/ml, IC50= 1.491 µg/ml
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- ELISA binding of mouse CTLA4 antibody MCT.E9 with mouse CTLA4 recombinant protein. Coating antigen: CTLA4-Fc, 1 µg/ml. CTLA4 antibody dilution start from 1000 ng/ml, EC50 is 7.191 ng/ml
- Submitted by
- LSBio (provider)
- Main image
- Experimental details
- ELISA blocking of mouse CTLA4 antibody MCT.E9 against mouse CD86 recombinant protein binding with Mouse CTLA4 recombinant protein. Coating antigen: CD86, 0.5 µg/ml. Mouse CTLA4 final concentration: 2 µg /ml CTLA4 antibody dilution start from 50 µg/ml, IC50=0.1494 µg/ml
- Submitted by
- LSBio (provider)
- Main image
- Experimental details
- ELISA blocking of mouse CTLA4 antibody MCT.E9 against mouse CD80 recombinant protein binding with Mouse CTLA4 recombinant protein. Coating antigen: CD80, 0.5 µg/ml. Mouse CTLA4 final concentration: 0.5 µg /ml CTLA4 antibody dilution start from 50 µg/ml, IC50= 1.491 µg/ml
- Submitted by
- LSBio (provider)
- Main image
- Experimental details
- ELISA binding of mouse CTLA4 antibody MCT.E9 with mouse CTLA4 recombinant protein. Coating antigen: CTLA4-Fc, 1 µg/ml. CTLA4 antibody dilution start from 1000 ng/ml, EC50 is 7.191 ng/ml