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ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Immunocytochemistry [1]
- Flow cytometry [1]
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- Product number
- MAB3253-100 - Provider product page
- Provider
- R&D Systems
- Product name
- Human CTLA-4 Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from cell culture supernatant. Detects human CTLA-4 in direct ELISAs.
- Reactivity
- Human
- Host
- Rabbit
- Conjugate
- Unconjugated
- Antigen sequence
P16410- Isotype
- IgG
- Antibody clone number
- 2188B
- Vial size
- 100 ug
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- CTLA-4 in Human PBMCs. CTLA-4 was detected in immersion fixed human peripheral blood mononuclear cells (PBMCs) stimulated with PHA using Rabbit Anti-Human CTLA-4 Monoclonal Antibody (Catalog # MAB3253) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rabbit IgG Secondary Antibody (red; Catalog # NL004) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of CTLA-4 in NS0 Mouse Cell Line Transfected with Human CTLA-4 and eGFP by Flow Cytometry. NS0 mouse myeloma cell line transfected with human CTLA-4 and eGFP was stained with (A) Rabbit Anti-Human CTLA-4 Monoclonal Antibody (Catalog # MAB3253) or (B) Normal Rabbit IgG Control Antibody (Catalog # MAB1050) followed by APC-conjugated Goat anti-Rabbit IgG Secondary Antibody (Catalog # F0111). View our protocol for Staining Membrane-associated Proteins.
