Explore
Validate
Learn
Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- ELISA [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- NBP1-07015 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NBP1-07015, RRID:AB_1625279
- Product name
- Goat Polyclonal GST Epitope Tag Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Goat
- Host
- Goat
- Conjugate
- Biotin
- Isotype
- IgG
- Vial size
- 0.2 mg
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
No comments: Submit comment
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- ELISA: GST Epitope Tag Antibody [Biotin] [NBP1-07015] - ELISA results of purified Goat anti-GST Antibody Biotin Conjugated tested against purified GST. Each well was coated in duplicate with 1.0 ug of GST (p/n 000-001-200). The starting dilution of antibody was 5ug/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using 3 percent fish gelatin as blocking buffer, Streptavidin Peroxidase Conjugated and TMB substrate p/n TMBE-1000.
