Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [1]
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Validation data
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- Product number
- MAB6894 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Mouse Monoclonal Glutathione S-Transferase mu 1/GSTM1 Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects human Glutathione S-Transferase mu 1/GSTM1 in ELISAs.
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
Submitted references A hPSC-based platform to discover gene-environment interactions that impact human β-cell and dopamine neuron survival.
Zhou T, Kim TW, Chong CN, Tan L, Amin S, Sadat Badieyan Z, Mukherjee S, Ghazizadeh Z, Zeng H, Guo M, Crespo M, Zhang T, Kenyon R, Robinson CL, Apostolou E, Wang H, Xiang JZ, Evans T, Studer L, Chen S
Nature communications 2018 Nov 16;9(1):4815
Nature communications 2018 Nov 16;9(1):4815
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
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- Experimental details
- Detection of Human Glutathione S-Transferase mu 1/GS by Western Blot. Western blot shows Recombinant Human GSTM1 (Catalog # 6894-GT, 3 ng/lane) and lysates of human adrenal gland tissue and human stomach tissue. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human Glutathione S-Transferase mu 1/GS Monoclonal Antibody (Catalog # MAB6894) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Glutathione S-Transferase mu 1/GS at approximately 25 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.