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- Antibody Data
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- References [16]
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- Western blot [6]
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- Product number
- NB600-326 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB600-326, RRID:AB_10001229
- Product name
- Rabbit Polyclonal GST Epitope Tag Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 ml
- Concentration
- 1.0 mg/ml
- Storage
- Store at 4C. Do not freeze.
Submitted references Identification of novel PDEδ interacting proteins.
PTPN12/PTP-PEST Regulates Phosphorylation-Dependent Ubiquitination and Stability of Focal Adhesion Substrates in Invasive Glioblastoma Cells.
Selective HIF-1 Regulation under Nonhypoxic Conditions by the p42/p44 MAP Kinase Inhibitor PD184161.
Hypoxia-inducible factor-1 plays a role in phosphate-induced vascular smooth muscle cell calcification.
A fully synthetic and biochemically validated phosphatidyl inositol-3-phosphate hapten via asymmetric synthesis and native chemical ligation.
GogB is an anti-inflammatory effector that limits tissue damage during Salmonella infection through interaction with human FBXO22 and Skp1.
Thromboxane A2 modulates cisplatin-induced apoptosis through a Siva1-dependent mechanism.
Bi-modal regulation of a formin by srGAP2.
ANKRD13C acts as a molecular chaperone for G protein-coupled receptors.
Loss of NADPH quinone oxidoreductase in the prostate and enhanced serum levels of cytokine-induced neutrophil chemoattractant 2alpha in hormone-stimulated noble rats: potential role in prostatic intraepithelial neoplasia development.
Loss of NADPH quinone oxidoreductase in the prostate and enhanced serum levels of cytokine-induced neutrophil chemoattractant 2alpha in hormone-stimulated noble rats: potential role in prostatic intraepithelial neoplasia development.
A distinct pool of phosphatidylinositol 4,5-bisphosphate in caveolae revealed by a nanoscale labeling technique.
The human ubiquitin conjugating enzyme, UBE2E3, is required for proliferation of retinal pigment epithelial cells.
Proteolysis of the barley receptor-like protein kinase RPG1 by a proteasome pathway is correlated with Rpg1-mediated stem rust resistance.
The intracellular trafficking of the G protein-coupled receptor TPbeta depends on a direct interaction with Rab11.
Nm23-H2 interacts with a G protein-coupled receptor to regulate its endocytosis through an Rac1-dependent mechanism.
Küchler P, Zimmermann G, Winzker M, Janning P, Waldmann H, Ziegler S
Bioorganic & medicinal chemistry 2018 May 1;26(8):1426-1434
Bioorganic & medicinal chemistry 2018 May 1;26(8):1426-1434
PTPN12/PTP-PEST Regulates Phosphorylation-Dependent Ubiquitination and Stability of Focal Adhesion Substrates in Invasive Glioblastoma Cells.
Chen Z, Morales JE, Guerrero PA, Sun H, McCarty JH
Cancer research 2018 Jul 15;78(14):3809-3822
Cancer research 2018 Jul 15;78(14):3809-3822
Selective HIF-1 Regulation under Nonhypoxic Conditions by the p42/p44 MAP Kinase Inhibitor PD184161.
Jalouli M, Mokas S, Turgeon CA, Lamalice L, Richard DE
Molecular pharmacology 2017 Nov;92(5):510-518
Molecular pharmacology 2017 Nov;92(5):510-518
Hypoxia-inducible factor-1 plays a role in phosphate-induced vascular smooth muscle cell calcification.
Mokas S, Larivière R, Lamalice L, Gobeil S, Cornfield DN, Agharazii M, Richard DE
Kidney international 2016 Sep;90(3):598-609
Kidney international 2016 Sep;90(3):598-609
A fully synthetic and biochemically validated phosphatidyl inositol-3-phosphate hapten via asymmetric synthesis and native chemical ligation.
Chandler BD, Burkhardt AL, Foley K, Cullis C, Driscoll D, Roy D'Amore N, Miller SJ
Journal of the American Chemical Society 2014 Jan 8;136(1):412-8
Journal of the American Chemical Society 2014 Jan 8;136(1):412-8
GogB is an anti-inflammatory effector that limits tissue damage during Salmonella infection through interaction with human FBXO22 and Skp1.
Pilar AV, Reid-Yu SA, Cooper CA, Mulder DT, Coombes BK
PLoS pathogens 2012;8(6):e1002773
PLoS pathogens 2012;8(6):e1002773
Thromboxane A2 modulates cisplatin-induced apoptosis through a Siva1-dependent mechanism.
Iorio-Morin C, Germain P, Roy S, Génier S, Labrecque P, Parent JL
Cell death and differentiation 2012 Aug;19(8):1347-57
Cell death and differentiation 2012 Aug;19(8):1347-57
Bi-modal regulation of a formin by srGAP2.
Mason FM, Heimsath EG, Higgs HN, Soderling SH
The Journal of biological chemistry 2011 Feb 25;286(8):6577-86
The Journal of biological chemistry 2011 Feb 25;286(8):6577-86
ANKRD13C acts as a molecular chaperone for G protein-coupled receptors.
Parent A, Roy SJ, Iorio-Morin C, Lépine MC, Labrecque P, Gallant MA, Slipetz D, Parent JL
The Journal of biological chemistry 2010 Dec 24;285(52):40838-51
The Journal of biological chemistry 2010 Dec 24;285(52):40838-51
Loss of NADPH quinone oxidoreductase in the prostate and enhanced serum levels of cytokine-induced neutrophil chemoattractant 2alpha in hormone-stimulated noble rats: potential role in prostatic intraepithelial neoplasia development.
Ghosh R, Schoolfield J, Yeh IT, Smith ML, Hursting SD, Chan DC, Lucia MS, Kumar AP
Translational oncology 2009 May;2(2):65-72
Translational oncology 2009 May;2(2):65-72
Loss of NADPH quinone oxidoreductase in the prostate and enhanced serum levels of cytokine-induced neutrophil chemoattractant 2alpha in hormone-stimulated noble rats: potential role in prostatic intraepithelial neoplasia development.
Ghosh R, Schoolfield J, Yeh IT, Smith ML, Hursting SD, Chan DC, Lucia MS, Kumar AP
Translational oncology 2009 May;2(2):65-72
Translational oncology 2009 May;2(2):65-72
A distinct pool of phosphatidylinositol 4,5-bisphosphate in caveolae revealed by a nanoscale labeling technique.
Fujita A, Cheng J, Tauchi-Sato K, Takenawa T, Fujimoto T
Proceedings of the National Academy of Sciences of the United States of America 2009 Jun 9;106(23):9256-61
Proceedings of the National Academy of Sciences of the United States of America 2009 Jun 9;106(23):9256-61
The human ubiquitin conjugating enzyme, UBE2E3, is required for proliferation of retinal pigment epithelial cells.
Plafker KS, Farjo KM, Wiechmann AF, Plafker SM
Investigative ophthalmology & visual science 2008 Dec;49(12):5611-8
Investigative ophthalmology & visual science 2008 Dec;49(12):5611-8
Proteolysis of the barley receptor-like protein kinase RPG1 by a proteasome pathway is correlated with Rpg1-mediated stem rust resistance.
Nirmala J, Dahl S, Steffenson BJ, Kannangara CG, von Wettstein D, Chen X, Kleinhofs A
Proceedings of the National Academy of Sciences of the United States of America 2007 Jun 12;104(24):10276-81
Proceedings of the National Academy of Sciences of the United States of America 2007 Jun 12;104(24):10276-81
The intracellular trafficking of the G protein-coupled receptor TPbeta depends on a direct interaction with Rab11.
Hamelin E, Thériault C, Laroche G, Parent JL
The Journal of biological chemistry 2005 Oct 28;280(43):36195-205
The Journal of biological chemistry 2005 Oct 28;280(43):36195-205
Nm23-H2 interacts with a G protein-coupled receptor to regulate its endocytosis through an Rac1-dependent mechanism.
Rochdi MD, Laroche G, Dupré E, Giguère P, Lebel A, Watier V, Hamelin E, Lépine MC, Dupuis G, Parent JL
The Journal of biological chemistry 2004 Apr 30;279(18):18981-9
The Journal of biological chemistry 2004 Apr 30;279(18):18981-9
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: GST Epitope Tag Antibody [NB600-326] - 0.1, 0.3, or 1.0.ug of 293 cell lysate expressing a GST-tag fusion protein. Antibody used at 0.06 ug/ml (1:15,000).
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Simple Western: GST Epitope Tag Antibody [NB600-326] - Simple Western lane view shows a specific band for GST Epitope Tag in 5 ng/ml of hVAV1-GST, E. coli (left) & hPARK2-GST-conditioned media (right) lysate(s). This experiment was performed under reducing conditions using the 12 - 230 kDa separation system.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Simple Western: GST Epitope Tag Antibody [NB600-326] - Simple Western lane view shows a specific band for GST Epitope Tag in 5 ng/ml of hUbiquitn-GST (left) & hISG15-GST (right) lysate(s). This experiment was performed under reducing conditions using the 12 - 230 kDa separation system.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Simple Western: GST Epitope Tag Antibody [NB600-326] - Electropherogram image(s) of corresponding Simple Western lane view. GST Epitope Tag antibody was used at 1:2000 dilution on hVAV1-GST, E. coli (left) & hPARK2-GST-conditioned media (right) lysate(s).
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Simple Western: GST Epitope Tag Antibody [NB600-326] - Electropherogram image(s) of corresponding Simple Western lane view. GST Epitope Tag antibody was used at 1:2000 dilution on hUbiquitn-GST (left) & hISG15-GST (right) lysate(s).
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: GST Epitope Tag Antibody [NB600-326] - Analysis using the HRP conjugate of NB600-326. Detection of 1.0, 0.3, or 0.1 ug of 293 cell lysate expressing a GST-tag fusion protein. Antibody used at 0.2 ug/ml (1:5,000).
