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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunohistochemistry [2]
- Flow cytometry [2]
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- Product number
- MA5-33004 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- GSTM1 Monoclonal Antibody (11F2)
- Antibody type
- Monoclonal
- Antigen
- Synthetic peptide
- Description
- Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.
- Reactivity
- Human, Mouse, Rat
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 11F2
- Vial size
- 100 µg
- Concentration
- 500 µg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of GSTM1 in Lane 1: human HeLa, whole cell lysate, Lane 2: human T-47D whole cell lysate, Lane 3: rat brain tissue lysate, Lane 4: rat lung tissue lysate, Lane 5: rat stomach tissue lysate, Lane 6: mouse lung tissue lysate, Lane 7: mouse stomach tissue lysate, Lane 8: mouse kidney tissue lysate. Electrophoresis was performed with 5-20% SDS-PAGE gel (70V, Stacking gel; 90V Resolving gel, Time: 2-3 hours), transferred to a nitrocellulose membrane and blocked using 5% Non-fat Milk/TBS (1.5 hrs at room temperature). Samples were incubated with GSTM1 monoclonal antibody (Product # MA5-33004) using a 0.5 µg/mL dilution, followed by a goat anti-mouse IgG-HRP at a dilution of 1:10,000, and developed with enhanced chemiluminescence (ECL).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of GSTM1 in, Lane 1: human Hela, whole cell lysate, Lane 2: human T-47D whole cell lysate, Lane 3: rat brain tissue lysate, Lane 4: rat lung tissue lysate, Lane 5: rat stomach tissue lysate, Lane 6: mouse lung tissue lysate, Lane 7: mouse stomach tissue lysate, Lane 8: mouse kidney tissue lysate. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50 µg of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. The membrane was blocked with 5% Non-fat Milk/ TBS for 1. 5 hour at RT. The membrane was incubated with GSTM1 Monoclonal Antibody (11F2) (Product # MA5-33004) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0. 1% Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1. 5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit. A specific band was detected for GSTM1 at approximately 26KD. The expected band size for GSTM1 is at 26KD.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of GSTM1 in paraffin-embedded rat liver tissue. Antigen retrieval was performed on the tissue using citrate buffer (pH 6, 20 min) and blocked with 10% goat serum. Samples were incubated with GSTM1 monoclonal antibody (Product # MA5-33004) at a 2 µg/mL dilution, followed by biotinylated goat anti-mouse IgG, and developed with Strepavidin-Biotin-Complex and DAB.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of GSTM1 in paraffin-embedded human colon cancer tissue. Antigen retrieval was performed on the tissue using citrate buffer (pH 6, 20 min) and blocked with 10% goat serum. Samples were incubated with GSTM1 monoclonal antibody (Product # MA5-33004) at a 2 µg/mL dilution, followed by biotinylated goat anti-mouse IgG, and developed with Strepavidin-Biotin-Complex and DAB.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometry of GSTM1 in U20S cells (blue line), isotype control rabbit IgG (green line) and unlabeled (red line). Samples were blocked with 10% goat serum, incubated with GSTM1 monoclonal antibody (Product # MA5-33004) at a dilution of 1 µg (per 1x10^6 cells), followed by 488 conjugated goat anti-mouse IgG (30 min at 20°C) using a 5-10 µg (per 1x10^6 cells) dilution.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometry of GSTM1 in HeLa cells (blue line), isotype control rabbit IgG (green line) and unlabeled (red line). Samples were blocked with 10% goat serum, incubated with GSTM1 monoclonal antibody (Product # MA5-33004) at a dilution of 1 µg (per 1x10^6 cells), followed by 488 conjugated goat anti-mouse IgG (30 min at 20°C) using a 5-10 µg (per 1x10^6 cells) dilution.
