PA5-22278

antibody from Invitrogen Antibodies

Targeting: GSTM1 GST1, H-B, MU

Antibody data

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Validation data

Product number: PA5-22278 - Provider product page
Provider: Invitrogen Antibodies
Product name: GSTM1 Polyclonal Antibody
Antibody type: Polyclonal
Antigen: Recombinant protein fragment
Description: Recommended positive controls: Human liver, Rat liver, Mouse brain. Predicted reactivity: Mouse (80%), Rat (82%), Bovine (83%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Reactivity: Human, Mouse, Rat
Host: Rabbit
Isotype: IgG
Vial size: 100 µL
Concentration: 1 mg/mL
Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

GSTM1 protein structure - PA5-22278 shown in red.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western Blot using GSTM1 Polyclonal Antibody (Product # PA5-22278). Human tissue extract (30 µg) was separated by 12% SDS-PAGE, and the membrane was blotted with GSTM1 Polyclonal Antibody (Product # PA5-22278) diluted at 1:1,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: GSTM1 Polyclonal Antibody detects GSTM1 protein by western blot analysis. A. 50 µg rat liver lysate/extract.12% SDS-PAGE. GSTM1 Polyclonal Antibody (Product # PA5-22278) dilution: 1:2,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: GSTM1 Polyclonal Antibody detects protein by western blot analysis. A. 50 µg mouse brain lysate/extract.12% SDS-PAGE. GSTM1 Polyclonal Antibody (Product # PA5-22278) dilution: 1:1,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis was performed on whole cell extract (30 µg lysate) of PC-12 (Lane 1), tissue extracts of Mouse Liver (Lane 2), Rat Liver (Lane 3), Mouse Kidney (Lane 4), Mouse Lung (Lane 5), Mouse Skeletal Muscle (Lane 6) and Mouse Uterus (Lane 7). The blot was probed with Anti-GSTM1 Polyclonal Antibody (Product # PA5-22278, 1:1000 dilution) and detected by chemiluminescence using Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.25 µg/mL, 1:4000 dilution). A 30 kDa band corresponding to GSTM1 was observed in PC-12 and also in Mouse Liver, Rat Liver, Mouse Kidney, Mouse Lung compared to other tissue extracts which are reported to be negative for GSTM1 expression.