NB600-446

antibody from Novus Biologicals

Targeting: GSTM1 GST1, H-B, MU

Western blot (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Antibody Data

Product number

NB600-446

Provider

Novus Biologicals

Proper citation

Novus Cat#NB600-446, RRID:AB_10003107

Product name

Mouse Monoclonal GST Epitope Tag Antibody

Antibody type

Monoclonal

Description

Protein G purified. Recognizes Glutathione S-Transferase (GST), a 26-kDa protein encoded by the parasitic helminth Schistosoma japonicum.

Reactivity

Human

Host

Mouse

Isotype

IgG

Vial size

100 ul

Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

References

Submitted references

The F-BAR Domain of Rga7 Relies on a Cooperative Mechanism of Membrane Binding with a Partner Protein during Fission Yeast Cytokinesis.

Liu Y, McDonald NA, Naegele SM, Gould KL, Wu JQ
Cell reports 2019 Mar 5;26(10):2540-2548.e4

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Fine-tuning the orientation of the polarity axis by Rga1, a Cdc42 GTPase-activating protein.

Miller KE, Lo WC, Lee ME, Kang PJ, Park HO
Molecular biology of the cell 2017 Dec 15;28(26):3773-3788

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Grp94 Protein Delivers γ-Aminobutyric Acid Type A (GABAA) Receptors to Hrd1 Protein-mediated Endoplasmic Reticulum-associated Degradation.

Di XJ, Wang YJ, Han DY, Fu YL, Duerfeldt AS, Blagg BS, Mu TW
The Journal of biological chemistry 2016 Apr 29;291(18):9526-39

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The Rho-GEF Gef3 interacts with the septin complex and activates the GTPase Rho4 during fission yeast cytokinesis.

Wang N, Wang M, Zhu YH, Grosel TW, Sun D, Kudryashov DS, Wu JQ
Molecular biology of the cell 2015 Jan 15;26(2):238-55

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Cooperation between Rho-GEF Gef2 and its binding partner Nod1 in the regulation of fission yeast cytokinesis.

Zhu YH, Ye Y, Wu Z, Wu JQ
Molecular biology of the cell 2013 Oct;24(20):3187-204

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Assembly and architecture of precursor nodes during fission yeast cytokinesis.

Laporte D, Coffman VC, Lee IJ, Wu JQ
The Journal of cell biology 2011 Mar 21;192(6):1005-21

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Prolyl isomerase Pin1 acts as a switch to control the degree of substrate ubiquitylation.

Siepe D, Jentsch S
Nature cell biology 2009 Aug;11(8):967-72

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An alternative domain containing a leucine-rich sequence regulates nuclear cytoplasmic localization of protein 4.1R.

Luque CM, Pérez-Ferreiro CM, Pérez-Gonzalez A, Englmeier L, Koffa MD, Correas I
The Journal of biological chemistry 2003 Jan 24;278(4):2686-91

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: GST Epitope Tag Antibody (3G10/1B3) [NB600-446] - Analysis of extracted from transformed E. coli which expressing GST-tagged protein, using GST-tag antibody. A: Non-induction E. coli lysate B: Induction E. coli lysate 10% SDS PAGE; antibody diluted at 1:5000.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: GST Epitope Tag Antibody (3G10) [NB600-446] - A. Non-induced cell lysate from E. coli expressing GST-tagged proteins B. IPTG-induced cell lysate from E. coli expressing GST-tagged proteins 10 % SDS-PAGE GST tag antibody [3G10] dilution: 1:1000

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: GST Epitope Tag Antibody (3G10) [NB600-446] - A. 2 ug His-recombinant protein B. 2 ug GST-recombinant protein C. 1 ug GST-recombinant protein D. 0.5 ug GST-recombinant protein 10 % SDS-PAGE GST tag antibody [3G10] dilution: 1:5000