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- Product number
- PA5-13553 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Asporin Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 400 µL
- Concentration
- 2 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Submitted references Right Ventricle Has Normal Myofilament Function But Shows Perturbations in the Expression of Extracellular Matrix Genes in Patients With Tetralogy of Fallot Undergoing Pulmonary Valve Replacement.
Brayson D, Holohan SJ, Bardswell SC, Arno M, Lu H, Jensen HK, Tran PK, Barallobre-Barreiro J, Mayr M, Dos Remedios CG, Tsang VT, Frigiola A, Kentish JC
Journal of the American Heart Association 2020 Aug 18;9(16):e015342
Journal of the American Heart Association 2020 Aug 18;9(16):e015342
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of 293 cells using an ASPN polyclonal antibody (Product # PA5-13553) at a dilution of 1:10-50, followed by a fluor-conjugated goat anti-rabbit secondary antibody (green). Nuclei were stained with DAPI (blue).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of Asporin in formalin-fixed and paraffin-embedded human hepatocarcinoma. Samples were incubated with Asporin polyclonal antibody (Product # PA5-13553) which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 7 Validation of small leucine-rich proteoglycan (SLRP) gene and protein expression shows asporin to be upregulated in several myocardial samples from patients with repair of tetralogy of Fallot undergoing pulmonary valve replacement (rToF-PVR). A , The mRNA validation of gene expression was performed by quantitative polymerase chain reaction analysis for the genes encoding asporin ( ASPN ), lumican ( LUM ), and osteoglycin ( OGN ); n=7 per group. Each data point shows the result from 1 right ventricle (RV) donor or rToF-PVR patient. Values are expressed as mean+-SD, indicated P values were returned after performing the Mann-Whitney U test. The P values were unchanged if the single rToF-PVR outlier was removed and the data were analyzed using the Student unpaired t test. B , Protein expression analysis for these genes was performed by western blotting using antibodies probing for asporin, lumican, and osteoglycin. Ponceau S shows equal loading of the gel lanes. Densitometry was performed for quantitation of protein abundance relative to ponceau S; RV donor, n=7; rToF-PVR, n=5.
