PA5-18352

antibody from Invitrogen Antibodies

Targeting: ASPN FLJ20129, PLAP-1, SLRR1C

Western blot (Data presented on provider website)

Immunocytochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-18352

Provider

Invitrogen Antibodies

Product name

Asporin Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

This antibody is predicted to react with rat based on sequence homology. This antibody is tested in Peptide ELISA: antibody detection limit dilution 64,000.

Reactivity

Human, Mouse, Rat

Host

Goat

Isotype

IgG

Vial size

100 μg

Concentration

0.5 mg/mL

Storage

-20°C, Avoid Freeze/Thaw Cycles

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescence analysis of Asporin in HeLa cells using a Asporin monoclonal antibody (Product # PA5-18352) at 10 µg/mL for1hr. The cells were paraformaldehyde fixed and permeabilized with 0.15% Triton. Primary incubation was followed by Alexa Fluor 488 secondary antibody (4 µg/mL) showing nuclear membrane staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 µg/mL)followed by Alexa Fluor 488 secondary antibody (4 µg/mL).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent staining of (A and C) ATDC5 cells and (B and C) DAPI using Product # PA5-18352.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry analysis of Asporin using Asporin Polyclonal Antibody (Product # PA5-18352) in paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 µg/mL) followed by Alexa Fluor 488 secondary antibody (4 µg/mL), showing nuclear membrane staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 µg/mL) followed by Alexa Fluor 488 secondary antibody (4 µg/mL).