PA5-28124

antibody from Invitrogen Antibodies

Targeting: ASPN FLJ20129, PLAP-1, SLRR1C

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-28124

Provider

Invitrogen Antibodies

Product name

Asporin Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

Recommended positive controls: A549, H1299, HCT116, mouse liver, rat heart.

Concentration

0.43 mg/mL

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot analysis of Asporin was performed by separating 50 µg of mouse tissue extract by 10% SDS-PAGE. Proteins were transferred to a membrane and probed with a Asporin Polyclonal Antibody (Product # PA5-28124) at a dilution of 1:500.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Asporin Polyclonal Antibody detects Asporin protein by western blot analysis. A. 30 µg A549 whole cell lysate/extract. B. 30 µg H1299 whole cell lysate/extract. C. 30 µg HCT116 whole cell lysate/extract.10 % SDS-PAGE. Asporin Polyclonal Antibody (Product # PA5-28124) dilution: 1:1,000.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot analysis of Asporin was performed by separating 50 µg of rat tissue extract by 10% SDS-PAGE. Proteins were transferred to a membrane and probed with a Asporin Polyclonal Antibody (Product # PA5-28124) at a dilution of 1:500.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of Asporin in methanol-fixed HeLa cells using an Asporin polyclonal antibody (Product # PA5-28124) at a 1:200 dilution.