100-401-142

antibody from Rockland Immunochemicals, Inc.

Targeting: ALPI IAP

Western blot (Supportive data in Antibodypedia)

Antibody Data

Product number

100-401-142

Provider

Rockland Immunochemicals, Inc.

Proper citation

Rockland Cat#100-401-142, RRID:AB_219900

Product name

Anti-ALKALINE PHOSPHATASE (Human Intestine) (RABBIT) Antibody - 100-401-142

Antibody type

Polyclonal

Vial size

2 µl

References

Submitted references

Rab11a is required for apical protein localisation in the intestine.

Sobajima T, Yoshimura S, Iwano T, Kunii M, Watanabe M, Atik N, Mushiake S, Morii E, Koyama Y, Miyoshi E, Harada A
Biology open 2014 Dec 19;4(1):86-94

Western blot

Supportive validation

Submitted by

Rockland Immunochemicals, Inc. (provider)

Main image

Experimental details

Both the antiserum and IgG fractions of anti-Alkaline Phosphatase (Human Intestine) are shown to detect under reducing conditions of SDS-PAGE the 60,000 dalton enzyme in cellular extracts. Approximately 10 ug of total protein is loaded per lane. A 1:5,000 dilution of the primary antibody is used followed by detection using HRP Goat-a-Rabbit IgG [H&L] (611-1302) diluted 1:4,000 and color development using 4-CN substrate until sufficient color develops. Other detection systems will yield similar results.

Validation comment

Western Blot