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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunohistochemistry [1]
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- Product number
- AP13096PU-N - Provider product page
- Provider
- OriGene
- Product name
- Gremlin 1 (GREM1) (C-term) rabbit polyclonal antibody, Purified
- Antibody type
- Polyclonal
- Description
- Gremlin 1 (GREM1) (C-term) rabbit polyclonal antibody, Purified
- Host
- Rabbit
- Conjugate
- Unconjugated
- Epitope
- GREM1
- Antibody clone number
- NULL
- Vial size
- 400 µl
- Concentration
- lot specific
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Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- (LEFT)Western blot analysis of anti-Gremlin Pab in CEM cell line lysates (35ug/lane). Gremlin (arrow) was detected using the purified Pab. (RIGHT)Western blot analysis of anti-Gremlin Pab in mouse liver cell lysate. Gremlin (arrow) was detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- (TOP) Formalin-PBS and Bouin embedded paraffin human renal tissue pretreated with heat induced epitope retrieval Citrate buffer pH 6.0 in microwave. Dilution of primary antibody was 1:50. Detection method was ABC system or Vectastaint Elite VECTOR. (BUTTOM) Formalin-fixed and paraffin-embedded human skeletal muscle tissue reacted with GREMLIN Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
- Validation comment
- IHC
