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Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [2]
- Immunohistochemistry [1]
- Chromatin Immunoprecipitation [1]
- Other assay [1]
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- Product number
- PA5-29101 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- SETDB1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: A549, HeLa, HepG2, HCT116. Predicted reactivity: Mouse (83%), Rat (84%), Bovine (94%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Submitted references Silencing of retrotransposons by SETDB1 inhibits the interferon response in acute myeloid leukemia.
Cuellar TL, Herzner AM, Zhang X, Goyal Y, Watanabe C, Friedman BA, Janakiraman V, Durinck S, Stinson J, Arnott D, Cheung TK, Chaudhuri S, Modrusan Z, Doerr JM, Classon M, Haley B
The Journal of cell biology 2017 Nov 6;216(11):3535-3549
The Journal of cell biology 2017 Nov 6;216(11):3535-3549
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of SETDB1 using 30 µg of HeLa lysate. Samples were loaded onto a 5% SDS-PAGE gel and probed with a SETDB1 polyclonal antibody (Product # PA5-29101) at a dilution of 1:1000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using SETDB1 Polyclonal Antibody (Product # PA5-29101). HeLa whole cell and nuclear extracts (30 µg) were separated by 5% SDS-PAGE, and the membrane was blotted with SETDB1 Polyclonal Antibody (Product # PA5-29101) diluted at 1:1,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- SETDB1 Polyclonal Antibody detects SETDB1 protein at nucleus by immunohistochemical analysis. Sample: Paraffin-embedded mouse testis. SETDB1 stained by SETDB1 Polyclonal Antibody (Product # PA5-29101) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Cross-linked ChIP was performed with HeLa chromatin extract and 5 µg of either control rabbit IgG or SETDB1 Polyclonal Antibody (Product # PA5-29101). The precipitated DNA was detected by PCR with primer set targeting to p53P2.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- SETDB1 Polyclonal Antibody immunoprecipitates SETDB1 protein in IP experiments. IP samples: HeLa nuclear extract. A. Control with 4 µg of preimmune Rabbit IgG. B. Immunoprecipitation of SETDB1 protein by 4 µg SETDB1 Polyclonal Antibody (Product # PA5-29101). 5 % SDS-PAGE. The immunoprecipitated SETDB1 protein was detected by SETDB1 Polyclonal Antibody (Product # PA5-29101) diluted at 1:1,000.
