Explore
Validate
Learn
Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [2]
Submit
Validation data
Reference
Comment
Report error
- Product number
- AF5905 - Provider product page
- Provider
- R&D Systems
- Product name
- Human Alkaline Phosphatase/ALPP/ALPI Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects recombinant human Alkaline Phosphatase Placental Type/ALPP and recombinant human Alkaline Phosphatase Intestinal Type/ALPI in direct ELISAs. Detects human ALPP and ALPI but not Alkaline Phosphatase Liver Type/ALPL in Western blots.
- Reactivity
- Human
- Host
- Sheep
- Conjugate
- Unconjugated
- Antigen sequence
P05187- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Structure-function analysis of Nel, a thrombospondin-1-like glycoprotein involved in neural development and functions.
Nakamura R, Nakamoto C, Obama H, Durward E, Nakamoto M
The Journal of biological chemistry 2012 Jan 27;287(5):3282-91
The Journal of biological chemistry 2012 Jan 27;287(5):3282-91
No comments: Submit comment
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Human Alkaline Phosphatase/ALPP/ALPI by Western Blot. Western blot shows lysates of human placenta tissue and JEG-3 human epithelial choriocarcinoma cell line. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human Alkaline Phosphatase/ALPP/ALPI Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5905) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Alkaline Phosphatase/ALPP/ALPI at approximately 68 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Human Alkaline Phosphatase/ALPP/ALPI by Simple WesternTM. Simple Western lane view shows lysates of human placenta tissue, loaded at 0.2 mg/mL. A specific band was detected for Alkaline Phosphatase/ALPP/ALPI at approximately 76 kDa (as indicated) using 10 µg/mL of Sheep Anti-Human Alkaline Phosphatase/ALPP/ALPI Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5905) followed by 1:50 dilution of HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
