GTX48822

antibody from GeneTex

Targeting: PDCD1 CD279, hSLE1, PD-1, PD1, SLEB2

Western blot (Supportive data in Antibodypedia)

Antibody Data

Product number

GTX48822

Provider

GeneTex

Proper citation

GeneTex Cat#GTX48822, RRID:AB_11172193

Product name

PDCD1 antibody [4F12]

Antibody type

Monoclonal

Host

Mouse

Vial size

100 µl

Western blot

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Western blot of SUMO-GFP fusion proteins cleaved by insect cell protein extracts.Anti-SUMO antibody, generated by immunization with recombinant yeast SUMO, was tested by western blot against several constructs of SUMO-GFP fusion proteins after cleavage by proteases in insect cell protein extracts. These constructs contained various linkers between the SUMO and GFP portion of the fusion proteins. Each sample was run twice. The left lanes each contain 2 µg E.coli expressed and purified SUMO-GFP fusion proteins after incubation with lysed cells (50 µg total protein) for 1 h. The right lanes contain the same fusion proteins incubated with the lysate in the presence of 2% SDS. After probing with anti-GFP antibodies the membranes were stripped of antibody using SDS-DTT solution for 30 m at 60° C and were then re-probed using the anti-SUMO antibody at a 1:1000 dilution incubated overnight at 4° C in 5% non-fat dry milk in TTBS. Detection occurred using a 1:2000 dilution of HRP-labeled Donkey anti-Rabbit IgG

Validation comment

WB