NBP2-34497

antibody from Novus Biologicals

Targeting: PDCD1 CD279, hSLE1, PD-1, PD1, SLEB2

Flow cytometry (Supportive data in Antibodypedia)

Functional assay (Data presented on provider website)

Antibody Data

Product number

NBP2-34497

Provider

Novus Biologicals

Product name

Armenian Hamster Monoclonal PD-1 Antibody

Antibody type

Monoclonal

Description

Protein A or G purified.

Reactivity

Mouse

Isotype

IgG

Vial size

0.1 mg

Concentration

1 mg/ml

Storage

Store at 4C. Do not freeze.

Flow cytometry

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Flow Cytometry: PD-1 Antibody (J43) [Serum/Azide Free] [NBP2-34497] - Analysis of mouse Con-A activated spleen cell suspensions and mouse PD-1 transfected cells using PD1 PE conjugate. This can be used at less than or equal to 0.5 ug per test. Cell number should be determined empirically but can range from 10^5to 10^8 cells

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Flow Cytometry: PD-1 Antibody (J43) [Serum/Azide Free] [NBP2-34497] - Staining of unstimulated (orange histogram) or 3-day Anti-Mouse CD3 and Anti-Mouse CD28 Functional Grade Purified -stimulated (purple histogram) C57Bl/6 splenocytes with 0.5 ug of Anti-Mouse CD279 (PD-1) FITC. Total viable cells were used for analysis.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Flow Cytometry: PD-1 Antibody (J43) [Serum/Azide Free] [NBP2-34497] - Staining of ConA stimulated splenocytes with Anti-Mouse CD279 (PD-1) FITC (left) and PE (right). Appropriate isotype controls were used (open histogram). Total viable cells were used for analysis.