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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
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- Validations
- Western blot [1]
- Immunocytochemistry [2]
- Immunohistochemistry [1]
- Flow cytometry [2]
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- Product number
- TA806808 - Provider product page
- Provider
- OriGene
- Proper citation
- OriGene Cat#TA806808, RRID:AB_2628240
- Product name
- PD-1 (PDCD1) mouse monoclonal antibody, clone OTI8H8 (formerly 8H8)
- Antibody type
- Monoclonal
- Description
- PD-1 (PDCD1) mouse monoclonal antibody, clone OTI8H8 (formerly 8H8)
- Host
- Mouse
- Conjugate
- Unconjugated
- Epitope
- PDCD1
- Isotype
- IgG
- Antibody clone number
- OTI8H8
- Vial size
- 100 µl
- Concentration
- 1 mg/ml
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Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PDCD1 (RC210364, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PDCD1.
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunofluorescent staining of PDCD1(RC210364)-stable-transfected HEK293T cells (left) labeling PDCD1 with mouse monoclonal antibody TA806808 (green) and nucleus with Hoechst33342 (blue) . HEK293T cells serve as negative control (right). (1:100)
- Validation comment
- IF
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunocytochemistry staining of stable expression PD1 cells using anti-PDCD1 mouse monoclonal antibody (TA806808)(Left) . The rihgt is negative control.(1:100)(1:900)
- Validation comment
- IF
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Human tonsil using anti-PDCD1 mouse monoclonal antibody. (Heat-induced epitope retrieval by Tris-EDTA, pH8.0, TA806808)(1:150)
- Validation comment
- IHC
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Flow cytometric Analysis of stable expression PD1 cells using anti-PDCD1 antibody(TA806808) (Red) compared to a nonspecific negative control antibody (Blue).(1:50)
- Validation comment
- FC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- HEK293T cells transfected with either mouse PD1(MR227347) overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-PDCD1 antibody(TA806808),compared to a nonspecific negative control antibody (green),and then analyzed by f
- Validation comment
- FC
