GTX50053

antibody from GeneTex

Targeting: PDCD1 CD279, hSLE1, PD-1, PD1, SLEB2

Western blot (Supportive data in Antibodypedia)

ELISA (Data presented on provider website)

Immunocytochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

GTX50053

Provider

GeneTex

Proper citation

GeneTex Cat#GTX50053, RRID:AB_11173278

Product name

PD1 antibody [4F12]

Antibody type

Monoclonal

Reactivity

Human

Host

Mouse

Western blot

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Cell lysates of 293T and U937 (40ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human PDCD1 (1:500). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

WB analysis of various cell lysate using GTX50053 PD1 antibody [4F12].Lane 1 : 293T cell lysateLane 2 : Raji cell lysateDilution : 1:3000Loading : 40£gg

Immunocytochemistry

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

ICC/IF analysis of HeLa cells using GTX50053 PD1 antibody [4F12].Green : Primary antibodyBlue : cell nucleusDilution : 1:100

Flow cytometry

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

FACS analysis of HeLa cells using GTX50053 PD1 antibody [4F12].Red : Primary antibodyBlack : Isotype controlDilution : 2-5£gg for 1x10? cells