Explore
Validate
Learn
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [4]
- Comments [0]
- Validations
- Western blot [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- MAB1086-100 - Provider product page
- Provider
- R&D Systems
- Product name
- Human PD-1 Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects human PD-1 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human (rh) CD28, rhCTLA4, rhICOS, or recombinant mouse PD-1 is observed.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Unconjugated
- Antigen sequence
Q8IX89- Isotype
- IgG
- Antibody clone number
- 192106
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Lymph node migratory dendritic cells modulate HIV-1 transcription through PD-1 engagement.
Soluble co-signaling molecules predict long-term graft outcome in kidney-transplanted patients.
Splenic proliferative lymphoid nodules distinct from germinal centers are sites of autoantigen stimulation in immune thrombocytopenia.
Programmed Death-1: from gene to protein in autoimmune human myasthenia gravis.
Banga R, Rebecchini C, Procopio FA, Noto A, Munoz O, Ioannidou K, Fenwick C, Ohmiti K, Cavassini M, Corpataux JM, de Leval L, Pantaleo G, Perreau M
PLoS pathogens 2019 Jul;15(7):e1007918
PLoS pathogens 2019 Jul;15(7):e1007918
Soluble co-signaling molecules predict long-term graft outcome in kidney-transplanted patients.
Melendreras SG, Martínez-Camblor P, Menéndez A, Bravo-Mendoza C, González-Vidal A, Coto E, Díaz-Corte C, Ruiz-Ortega M, López-Larrea C, Suárez-Álvarez B
PloS one 2014;9(12):e113396
PloS one 2014;9(12):e113396
Splenic proliferative lymphoid nodules distinct from germinal centers are sites of autoantigen stimulation in immune thrombocytopenia.
Daridon C, Loddenkemper C, Spieckermann S, Kühl AA, Salama A, Burmester GR, Lipsky PE, Dörner T
Blood 2012 Dec 13;120(25):5021-31
Blood 2012 Dec 13;120(25):5021-31
Programmed Death-1: from gene to protein in autoimmune human myasthenia gravis.
Sakthivel P, Ramanujam R, Wang XB, Pirskanen R, Lefvert AK
Journal of neuroimmunology 2008 Jan;193(1-2):149-55
Journal of neuroimmunology 2008 Jan;193(1-2):149-55
No comments: Submit comment
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Human PD-1 by Western Blot. Western blot shows lysates of human peripheral blood mononuclear cells (PBMC) untreated (-) or treated (+) with 1 µg/mL PHA for 5 days. PVDF Membrane was probed with 1 µg/mL of Human PD-1 Monoclonal Antibody (Catalog # MAB1086) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for PD-1 at approximately 33 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
