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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [2]
- Immunohistochemistry [1]
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- Product number
- ARG56131 - Provider product page
- Provider
- Arigo
- Product name
- anti-PD-1 / CD279 antibody [4D6]
- Antibody type
- Monoclonal
- Antigen
- Extracellular domain of Human PD-1
- Description
- Purification with Protein A.
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 4D6
- Vial size
- 50 µg
- Concentration
- 1 mg/ml
- Storage
- For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening.
- Handling
- The antibody solution should be gently mixed before use.
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Supportive validation
- Submitted by
- Arigo (provider)
- Main image
- Experimental details
- Western blot: Transfected 293 cell lysate stained with ARG56131 anti-PD-1 / CD279 antibody [4D6] at (A) 0.25 and (B) 0.5 µg/ml dilution.
Supportive validation
- Submitted by
- Arigo (provider)
- Main image
- Experimental details
- Immunocytochemistry: Transfected 293 cells stained with ARG56131 anti-PD-1 / CD279 antibody [4D6] at 5 µg/ml dilution. Lower left: Same cells stained with control Mouse IgG antibody at 5 µg/ml dilution.
- Submitted by
- Arigo (provider)
- Main image
- Experimental details
- Immunocytochemistry: Transfected 293 cells stained with ARG56131 anti-PD-1 / CD279 antibody [4D6] at 5 µg/ml dilution. Lower left: Same cells stained with control Mouse IgG antibody at 5 µg/ml dilution.
Supportive validation
- Submitted by
- Arigo (provider)
- Main image
- Experimental details
- Immunohistochemistry: (A) Human tonsil tissue, (B) Human lymph node tissue, and (C) Human spleen tissue stained with ARG56131 anti-PD-1 / CD279 antibody [4D6] at 5 µg/ml dilution. (D) Human tonsil tissue stained with control Mouse IgG antibody at 5 µg/ml dilution.
