CF807889

antibody from Invitrogen Antibodies

Targeting: PDCD1 CD279, hSLE1, PD-1, PD1, SLEB2

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

CF807889

Provider

Invitrogen Antibodies

Product name

PDCD1 Monoclonal Antibody (OTI3A1), TrueMAB™

Antibody type

Monoclonal

Antigen

Recombinant full-length protein

Reactivity

Human, Mouse

Host

Mouse

Isotype

IgG

Antibody clone number

OTI3A1

Vial size

100 µg

Concentration

1 mg/mL

Storage

-20° C, Avoid Freeze/Thaw Cycles

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PDCD1 (RC210364, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 µg per lane) were separated by SDS-PAGE and immunoblotted with anti-PDCD1. Positive lysates LY401555 (100 µg) and LC401555 (20 µg) can be purchased separately from OriGene.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent staining of PDCD1(RC210364)-stable-transfected HEK293T cells (left) labeling PDCD1 with mouse monoclonal antibody TA807889 (green) and nucleus with Hoechst33342 (blue). HEK293T cells serve as negative control (right). (1:100)

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent staining of PDCD1(RC210364)-stable-transfected HEK293T cells (left) labeling PDCD1 with mouse monoclonal antibody TA807889 (green) and nucleus with Hoechst33342 (blue). HEK293T cells serve as negative control (right). (1:100)

Flow cytometry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Flow cytometric analysis of stable expression PD1 cells using anti-PDCD1 antibody (TA807889) (Red) compared to a nonspecific negative control antibody (Blue). (1:50)

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

HEK293T cells transfected with either mouse PD1(MR227347) overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-PDCD1 antibody (TA807889), compared to a nonspecific negative control antibody (green), and then analyzed by flow cytometry. (1:50)

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Flow cytometric analysis of stable expression PDL1 (RC213071) cells using anti-PDCD1 antibody (TA807889) (blue) or 0.3 µg/mL PD1-Fc fusion protein (TP700199) (green) or both (red),and detected by anti-Fc(human) IgG-FITC. (1:50)

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Flow cytometric analysis of PDL2 (RC224141) transilently transfected HEK293T cells using anti-PDCD1 antibody (TA807889) (blue) or 1 µg/mL PD1-Fc fusion protein (TP700199) (green) or both (red),and detected by anti-Fc(human) IgG-FITC. (1:50)