Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [5]
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Validation data
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- Product number
- NBP1-88104 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NBP1-88104, RRID:AB_11017958
- Product name
- Rabbit Polyclonal PD-1 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Specificity of human PD-1 antibody verified on a Protein Array containing target protein plus 383 other non-specific proteins.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Submitted references Immune response in breast cancer brain metastases and their microenvironment: the role of the PD-1/PD-L axis.
Duchnowska R, Pęksa R, Radecka B, Mandat T, Trojanowski T, Jarosz B, Czartoryska-Arłukowicz B, Olszewski WP, Och W, Kalinka-Warzocha E, Kozłowski W, Kowalczyk A, Loi S, Biernat W, Jassem J, Polish Brain Metastasis Consortium
Breast cancer research : BCR 2016 Apr 27;18(1):43
Breast cancer research : BCR 2016 Apr 27;18(1):43
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: PD-1 Antibody [NBP1-88104] - Analysis in control (vector only transfected HEK293T lysate) and pDCD1 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: PD-1 Antibody [NBP1-88104] - Staining of human lymph node shows strong cytoplasmic positivity in germinal center cells.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: PD-1 Antibody [NBP1-88104] - Staining of human tonsil shows strong cytoplasmic positivity in germinal center cells.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: PD-1 Antibody [NBP1-88104] - Staining of human liver shows no positivity in hepatocytes as expected.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: PD-1 Antibody [NBP1-88104] - Staining in human lymph node and liver tissues . Corresponding PDCD1 RNA-seq data are presented for the same tissues.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: PD-1 Antibody [NBP1-88104] - Staining of human placenta shows no positivity in trophoblastic cells as expected.