Antibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [4]
- Other assay [5]
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Validation data
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- Product number
- MA5-15780 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- PD-1 Monoclonal Antibody (7A11B1)
- Antibody type
- Monoclonal
- Antigen
- Recombinant full-length protein
- Description
- A suggested positive control is PD-1 recombinant protein.
- Reactivity
- Human, Mouse, Rat
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 7A11B1
- Vial size
- 100 µg
- Concentration
- 1 mg/mL
- Storage
- -20°C
Submitted references Knockdown of Leptin Receptor Affects Macrophage Phenotype in the Tumor Microenvironment Inhibiting Breast Cancer Growth and Progression.
Roles of programmed death protein 1/programmed death-ligand 1 in secondary brain injury after intracerebral hemorrhage in rats: selective modulation of microglia polarization to anti-inflammatory phenotype.
Gelsomino L, Naimo GD, Malivindi R, Augimeri G, Panza S, Giordano C, Barone I, Bonofiglio D, Mauro L, Catalano S, Andò S
Cancers 2020 Jul 27;12(8)
Cancers 2020 Jul 27;12(8)
Roles of programmed death protein 1/programmed death-ligand 1 in secondary brain injury after intracerebral hemorrhage in rats: selective modulation of microglia polarization to anti-inflammatory phenotype.
Wu J, Sun L, Li H, Shen H, Zhai W, Yu Z, Chen G
Journal of neuroinflammation 2017 Feb 14;14(1):36
Journal of neuroinflammation 2017 Feb 14;14(1):36
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Supportive validation
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- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of A-20 cell lysate using a CD279/PD1 monoclonal antibody (Product # MA5-15780) at 1 µg/mL in (A) the absence and (B) presence of blocking recombinant protein.
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- Invitrogen Antibodies (provider)
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- Experimental details
- Western Blot analysis of PD-1 in A-20 cell lysate with PD-1 Monoclonal Antibody (7A11B1) (Product # MA5-15780) at 1 µg/mL in the (A) absence and (B) presence of blocking recombinant protein.
Supportive validation
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- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence of PD-1 in human spleen tissue with PD-1 Monoclonal Antibody (7A11B1) (Product # MA5-15780) at 20 µg/mL. Green: PD-1 Antibody [7A11B1] (PM-5177) Blue: DAPI staining
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- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence of PD-1 in mouse brain tissue with PD-1 Monoclonal Antibody (7A11B1) (Product # MA5-15780) at 20 µg/mL.
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- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of PD-1 in human spleen tissue with PD-1 Monoclonal Antibody (7A11B1) (Product # MA5-15780) at 25 µg/mL.
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- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of PD-1 in mouse brain tissue with PD-1 Monoclonal Antibody (7A11B1) (Product # MA5-15780) at 2.5 µg/mL.
Supportive validation
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- Invitrogen Antibodies (provider)
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- Experimental details
- Fig. 2 ICH increased the protein levels of PD-1/PD-Ls and the interaction between PD-1 and PD-L1. a Time course of the protein levels of PD-1, PD-L1, and PD-L2 in the brain tissue around hematoma after ICH. Representative western blot bands of PD-1, PD-L1, and PD-L2 and quantitative analysis of the relative protein level were shown. The mean value of sham group was normalized to 1.0. Data are expressed as mean +- SEM, n = 6. Double asterisks indicate p < 0.01, triple asterisks indicate p < 0.001 vs. sham group. b , c Immunoprecipitation analysis of the interaction between PD-1 and PD-L1 at indicated times after ICH. All values are means +- SEM, n = 6. triple asterisks indicate p < 0.001 vs. sham group, triple pound signs indicate p < 0.001
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- Experimental details
- Fig. 4 Effects of PD-1/PD-L1 overexpression and knockdown on ICH-induced microglia polarization and STAT1 phosphorylation. a ICH rats accepted intracerebroventricular injection of pDNA or siRNAs as indicated. Sections were stained for CD16/CD11b (pro-inflammatory microglia marker) or CD206/CD11b (anti-inflammatory microglia marker). Percentage of CD16-positive cells or CD206-positive cells was shown in b and d and representative images were shown in c and e . Scale bar = 64 mum. In b and d , data are expressed as mean +- SEM, n = 6. Triple asterisks indicate p < 0.001 vs. sham group; single pound sign indicates p < 0.05, double pound signs indicate p < 0.01 vs. ICH + vector group; single ampersand indicates p < 0.05, double ampersands indicate p < 0.01 vs. ICH + control siRNA group. f Sections of ICH + PD-1 overexpression + PD-L1 overexpression group were stained for PD1/CD16/CD206. White arrows point to CD206-positive cells with high fluorescence intensity of PD-1, and purple arrows point to CD16-positive cells with low fluorescence intensity of PD-1. Scale bar = 64 mum. g Western blot analysis and quantification of the phosphorylation level of STAT1. The mean values of the protein levels in the sham group were normalized to 1.0. Data are expressed as mean +- SEM, n = 6. Double asterisks indicate p < 0.01, triple asterisks indicate p < 0.001 vs. sham group; double pound signs indicate p < 0.01, single ampersand indicates p < 0.05. h Western blot analysis and quantification o
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- Experimental details
- Fig. 5 Effects of PD-1/PD-L1 overexpression and knockdown on the polarization of OxyHb-treated microglia. a Cultured microglia accepted transfection of pDNA or siRNAs as indicated. b The efficiency of pDNA and siRNA in cultured microglia was verified by immunofluorescence staining. Scale bar = 64 mum. Cultured microglia was stained for CD16/CD11b (pro-inflammatory microglia marker) or CD206/CD11b (anti-inflammatory microglia marker). Percentage of CD16-positive cells or CD206-positive cells was shown in c and e and representative images were shown in d and f . Scale bar = 64 mum. In c and e , data are expressed as mean +- SEM, n = 6. Single asterisk indicates p < 0.05, triple asterisks indicate p < 0.001 vs. sham group; single pound sign indicates p < 0.05, double pound signs indicate p < 0.01 vs. ICH + vector group; single ampersand indicates p < 0.05, double ampersands indicate p < 0.01 vs. ICH + control siRNA group. g Microglia of ICH + PD-1 overexpression + PD-L1 overexpression group was stained for PD1/CD16/CD206. White arrows point to CD206-positive cells with high fluorescence intensity of PD-1, and purple arrows point to CD16-positive cells with low fluorescence intensity of PD-1. Scale bar = 64 mum
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- Experimental details
- Fig. 3 Effects of PD-1/PD-L1 overexpression and knockdown on brain cell death and neuronal degeneration after ICH. a ICH rats accepted intracerebroventricular injection of pDNA or siRNAs as indicated. b Western blot analysis of the efficiency of PD-1 and PD-L1 overexpression or knockdown in brain of ICH rats. Quantification of relative protein levels of PD-1 and PD-L1 was shown in c. c Data are expressed as mean +- SEM, n = 6. Triple asterisks indicate p < 0.001 vs. ICH + vector group; triple pound signs indicate p < 0.001 vs. ICH + control siRNA group. d Terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) staining. Sections were labeled by TUNEL ( green ) to detect apoptotic brain cells and counterstained with DAPI ( blue ) to detect nuclei. Arrows point to TUNEL-positive cells. Scale bar = 64 mum. e Percentage of TUNEL-positive cells. Data are expressed as mean +- SEM, n = 6. Triple asterisks indicate p < 0.001 vs. sham group; double pound signs indicate p < 0.01 vs. ICH + vector group; ampersand indicates p < 0.05, double ampersands indicate p < 0.01 vs. ICH + control siRNA group. f Fluoro-jade B (FJB) staining. Arrows point to FJB-positive cells. Scale bar = 64 mum. The number of FJB-positive brain cells was calculated. g Data are expressed as mean +- SEM, n = 6. Triple asterisks indicate p < 0.001 vs. sham group; double pound signs indicate p < 0.01 vs. ICH + vector group; double ampersands indicate p < 0.01, triple ampersands indicate p < 0.001 vs. ICH
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- Figure 7 Effects of the lack of ObR on the tumor immune microenvironment. ( A ) Immunoblotting showing programmed death ligand 1 (PD-L1) protein expression in Control sh and ObR sh MCF-7 and MDA-MB-231 breast cancer cells. beta-Actin was used as a control for equal loading and transfer. Italicized numbers below blots represent the mean of the band optical density expressed as fold over Control sh ObR cells. PD-L1, programmed cell death protein (PD-1) and arginase (ARG 1) immunohistochemical staining and relative score of Control sh and ObR sh MCF-7 ( B ) and MDA-MB-231 ( C ) xenograft tumor sections. Inset, negative control. Scale bar = 25 mum. THP-1 cells were stimulated with phorbol 12-myristate 13-acetate (PMA, 100 nM) for 14 h followed by 24 h rest to obtain THP-1 macrophage-like cells (M0). Phagocytic activity of M0 treated with 5% charcoal stripped media (-) or incubated with conditioned media (CM) derived from control sh and ObR sh MCF-7 breast cancer cells for 5 days ( D ) and MDA-MB-231 for 3 days ( E ). Cells were incubated with latex beads conjugated with FITC-IgG for 2 h. Pixel intensity of FITC labeled beads was normalized to number of cells and results are expressed as percentage. The values represent the mean +- SD of three different experiments, each performed in triplicate. n.s., nonsignificant; * p < 0.05; ** p < 0.005.