Antibody data
- Antibody Data
- Antigen structure
- References [11]
- Comments [0]
- Validations
- Flow cytometry [1]
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- Product number
- BAF1086 - Provider product page
- Provider
- R&D Systems
- Product name
- Human PD-1 Biotinylated Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects human PD-1 in ELISAs and Western blots. In sandwich ELISAs, less than 2% cross-reactivity with recombinant mouse PD-1 is observed and less than 0.2% cross-reactivity with recombinant human (rh) CD28, rhICOS, and rhCTLA-4 is observed.
- Reactivity
- Human
- Host
- Goat
- Conjugate
- Biotin
- Antigen sequence
Q8IX89
- Isotype
- IgG
- Vial size
- 50 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Evaluation of costimulatory molecules in dogs with B cell high grade lymphoma.
Treatment with native heterodimeric IL-15 increases cytotoxic lymphocytes and reduces SHIV RNA in lymph nodes.
Immunohistochemical Analysis of PD-L1 Expression in Canine Malignant Cancers and PD-1 Expression on Lymphocytes in Canine Oral Melanoma.
Type I interferon responses in rhesus macaques prevent SIV infection and slow disease progression.
Loss of circulating CD4 T cells with B cell helper function during chronic HIV infection.
Programmed death 1-mediated T cell exhaustion during visceral leishmaniasis impairs phagocyte function.
CD4 T follicular helper cell dynamics during SIV infection.
Distribution, persistence, and efficacy of adoptively transferred central and effector memory-derived autologous simian immunodeficiency virus-specific CD8+ T cell clones in rhesus macaques during acute infection.
Trafficking, persistence, and activation state of adoptively transferred allogeneic and autologous Simian Immunodeficiency Virus-specific CD8(+) T cell clones during acute and chronic infection of rhesus macaques.
Activation drives PD-1 expression during vaccine-specific proliferation and following lentiviral infection in macaques.
Programmed Death-1: from gene to protein in autoimmune human myasthenia gravis.
Tagawa M, Kurashima C, Takagi S, Maekawa N, Konnai S, Shimbo G, Matsumoto K, Inokuma H, Kawamoto K, Miyahara K
PloS one 2018;13(7):e0201222
PloS one 2018;13(7):e0201222
Treatment with native heterodimeric IL-15 increases cytotoxic lymphocytes and reduces SHIV RNA in lymph nodes.
Watson DC, Moysi E, Valentin A, Bergamaschi C, Devasundaram S, Fortis SP, Bear J, Chertova E, Bess J Jr, Sowder R, Venzon DJ, Deleage C, Estes JD, Lifson JD, Petrovas C, Felber BK, Pavlakis GN
PLoS pathogens 2018 Feb;14(2):e1006902
PLoS pathogens 2018 Feb;14(2):e1006902
Immunohistochemical Analysis of PD-L1 Expression in Canine Malignant Cancers and PD-1 Expression on Lymphocytes in Canine Oral Melanoma.
Maekawa N, Konnai S, Okagawa T, Nishimori A, Ikebuchi R, Izumi Y, Takagi S, Kagawa Y, Nakajima C, Suzuki Y, Kato Y, Murata S, Ohashi K
PloS one 2016;11(6):e0157176
PloS one 2016;11(6):e0157176
Type I interferon responses in rhesus macaques prevent SIV infection and slow disease progression.
Sandler NG, Bosinger SE, Estes JD, Zhu RT, Tharp GK, Boritz E, Levin D, Wijeyesinghe S, Makamdop KN, del Prete GQ, Hill BJ, Timmer JK, Reiss E, Yarden G, Darko S, Contijoch E, Todd JP, Silvestri G, Nason M, Norgren RB Jr, Keele BF, Rao S, Langer JA, Lifson JD, Schreiber G, Douek DC
Nature 2014 Jul 31;511(7511):601-5
Nature 2014 Jul 31;511(7511):601-5
Loss of circulating CD4 T cells with B cell helper function during chronic HIV infection.
Boswell KL, Paris R, Boritz E, Ambrozak D, Yamamoto T, Darko S, Wloka K, Wheatley A, Narpala S, McDermott A, Roederer M, Haubrich R, Connors M, Ake J, Douek DC, Kim J, Petrovas C, Koup RA
PLoS pathogens 2014 Jan;10(1):e1003853
PLoS pathogens 2014 Jan;10(1):e1003853
Programmed death 1-mediated T cell exhaustion during visceral leishmaniasis impairs phagocyte function.
Esch KJ, Juelsgaard R, Martinez PA, Jones DE, Petersen CA
Journal of immunology (Baltimore, Md. : 1950) 2013 Dec 1;191(11):5542-50
Journal of immunology (Baltimore, Md. : 1950) 2013 Dec 1;191(11):5542-50
CD4 T follicular helper cell dynamics during SIV infection.
Petrovas C, Yamamoto T, Gerner MY, Boswell KL, Wloka K, Smith EC, Ambrozak DR, Sandler NG, Timmer KJ, Sun X, Pan L, Poholek A, Rao SS, Brenchley JM, Alam SM, Tomaras GD, Roederer M, Douek DC, Seder RA, Germain RN, Haddad EK, Koup RA
The Journal of clinical investigation 2012 Sep;122(9):3281-94
The Journal of clinical investigation 2012 Sep;122(9):3281-94
Distribution, persistence, and efficacy of adoptively transferred central and effector memory-derived autologous simian immunodeficiency virus-specific CD8+ T cell clones in rhesus macaques during acute infection.
Minang JT, Trivett MT, Bolton DL, Trubey CM, Estes JD, Li Y, Smedley J, Pung R, Rosati M, Jalah R, Pavlakis GN, Felber BK, Piatak M Jr, Roederer M, Lifson JD, Ott DE, Ohlen C
Journal of immunology (Baltimore, Md. : 1950) 2010 Jan 1;184(1):315-26
Journal of immunology (Baltimore, Md. : 1950) 2010 Jan 1;184(1):315-26
Trafficking, persistence, and activation state of adoptively transferred allogeneic and autologous Simian Immunodeficiency Virus-specific CD8(+) T cell clones during acute and chronic infection of rhesus macaques.
Bolton DL, Minang JT, Trivett MT, Song K, Tuscher JJ, Li Y, Piatak M Jr, O'Connor D, Lifson JD, Roederer M, Ohlen C
Journal of immunology (Baltimore, Md. : 1950) 2010 Jan 1;184(1):303-14
Journal of immunology (Baltimore, Md. : 1950) 2010 Jan 1;184(1):303-14
Activation drives PD-1 expression during vaccine-specific proliferation and following lentiviral infection in macaques.
Hokey DA, Johnson FB, Smith J, Weber JL, Yan J, Hirao L, Boyer JD, Lewis MG, Makedonas G, Betts MR, Weiner DB
European journal of immunology 2008 May;38(5):1435-45
European journal of immunology 2008 May;38(5):1435-45
Programmed Death-1: from gene to protein in autoimmune human myasthenia gravis.
Sakthivel P, Ramanujam R, Wang XB, Pirskanen R, Lefvert AK
Journal of neuroimmunology 2008 Jan;193(1-2):149-55
Journal of neuroimmunology 2008 Jan;193(1-2):149-55
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of PD-1 in Human PBMCs treated with PHA by Flow Cytometry. Human peripheral blood mononuclear cells (PBMCs) either (A) untreated or (B) treated with 5 μg/mL PHA overnight were stained with Goat Anti-Human PD-1 Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF1086) followed by Streptavidin-Phycoerythrin (Catalog # F0040) and Mouse Anti-Human CD3 epsilon APC-conjugated Monoclonal Antibody (Catalog # FAB100A). Quadrant markers were set based on control antibody staining (Catalog # BAF108). View our protocol for Staining Membrane-associated Proteins.