FAB7115G

antibody from R&D Systems

Targeting: PDCD1 CD279, hSLE1, PD-1, PD1, SLEB2

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

FAB7115G

Provider

R&D Systems

Product name

Human PD-1 Alexa Fluor® 488-conjugated Antibody

Antibody type

Polyclonal

Description

Antigen Affinity-purified. Detects human PD-1 in ELISAs and Western blots. In sandwich ELISAs, less than 2% cross-reactivity with recombinant mouse PD-1 and less than 0.2% cross-reactivity with recombinant human (rh) CD28, rhICOS, and rhCTLA-4 is observed.

Reactivity

Human

Host

Goat

Conjugate

Green dye

Antigen sequence

Q8IX89


Isotype

IgG

Vial size

100 Tests

Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied.

References

Submitted references

Lymphoid tissue fibrosis is associated with impaired vaccine responses.

Kityo C, Makamdop KN, Rothenberger M, Chipman JG, Hoskuldsson T, Beilman GJ, Grzywacz B, Mugyenyi P, Ssali F, Akondy RS, Anderson J, Schmidt TE, Reimann T, Callisto SP, Schoephoerster J, Schuster J, Muloma P, Ssengendo P, Moysi E, Petrovas C, Lanciotti R, Zhang L, Arévalo MT, Rodriguez B, Ross TM, Trautmann L, Sekaly RP, Lederman MM, Koup RA, Ahmed R, Reilly C, Douek DC, Schacker TW
The Journal of clinical investigation 2018 Jul 2;128(7):2763-2773

Flow cytometry

Supportive validation

Submitted by

R&D Systems (provider)

Main image

Experimental details

Detection of PD-1 in Human PBMCs treated with PHA by Flow Cytometry. Human peripheral blood mononuclear cells (PBMCs) either (A) untreated or (B) treated with 5 ug/mL PHA overnight were stained with Goat Anti-Human PD-1 Alexa Fluor® 488-conjugated Antigen Affinity-purified Polyclonal Antibody (Catalog # FAB7115G) and Mouse Anti-Human CD3 epsilon APC-conjugated Monoclonal Antibody (Catalog # FAB100A). Quadrant markers were set based on control antibody staining (Catalog # IC108G). View our protocol for Staining Membrane-associated Proteins.