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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
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- Product number
- BS1317 - Provider product page
- Provider
- Bioworld Technology, Inc
- Proper citation
- Bioworld Technology Cat#BS1317, RRID:AB_1662254
- Product name
- Rit1 (W204) polyclonal antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide, corresponding to amino acids 170-220 of Human Rit1.
- Description
- ROC 1 and ROC 2 are the two highly conserved RING finger proteins that are homologous to APC 11, a subunit of the anaphase promoting complex(APC). ROC 1 and ROC 2 commonly interact with all cullins while APC 11 specifically interacts with APC 2, a cullin related APC subunit. ROC 1 and APC 11 immunocomplexes can catalyze isopeptide ligations to form polyubiquitin chains in an E1 and E2 dependent manner. ROC 1 is thought to play a unique role in the ubiquitination reaction by heterodimerizing with cullin 1 to catalyze ubiquitin polymerization.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100ul
- Concentration
- 1 mg/ml
- Storage
- Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
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Supportive validation
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- Bioworld Technology, Inc (provider)
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- Experimental details
- Western blot (WB) analysis of Rit1 (W204) pAb at 1:500 dilutionLane1:HEK293T whole cell lysateLane2:sp2/0 whole cell lysateLane3:H9C2 whole cell lysate
