Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
- Other assay [1]
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Validation data
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- Product number
- PA5-30919 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- RIT1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: A431. Predicted reactivity: Mouse (97%), Zebrafish (87%), Xenopus laevis (87%), Pig (97%), Chicken (96%), Sheep (99%), Rhesus Monkey (100%), Bovine (98%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.75 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Submitted references Computational analysis of data from a genome-wide screening identifies new PARP1 functional interactors as potential therapeutic targets.
Lodovichi S, Mercatanti A, Cervelli T, Galli A
Oncotarget 2019 Apr 12;10(28):2722-2737
Oncotarget 2019 Apr 12;10(28):2722-2737
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using RIT1 Polyclonal Antibody (Product # PA5-30919). Sample (30 µg of whole cell lysate). Lane A: A431. 12% SDS PAGE. RIT1 Polyclonal Antibody (Product # PA5-30919) diluted at 1:1,000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- RIT1 Polyclonal Antibody detects RIT1 protein at cytosol on BT483 xenograft by immunohistochemical analysis. Sample: Paraffin-embedded BT4838 xenograft. RIT1 Polyclonal Antibody (Product # PA5-30919) dilution: 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 3 Effect of RIT1, INCENP and PSAT1 on PARylation and PARP1 protein level, and olaparip sensitivity (A) Expression of INCENP, RIT1 and PSAT1 in triple negative and hormone receptor positive (+) breast cancer cell lines: (B) Expression of INCENP, RIT1 and PSAT1 in high grade serous and ovarian clear cell cancer lines. Data were compared and statically analyzed by Student's t -test as indicated: ns, not significant, * p