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Flow cytometryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Flow cytometry [1]
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- Product number
- MAB4694 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Mouse Monoclonal Semaphorin 4A Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects human Semaphorin 4A in direct ELISAs. In direct ELISAs, 50%-100% cross-reactivity with recombinant human (rh) Semaphorin 4C and rhSemaphorin 4G is observed, and less than 5% cross-reactivity with rhSemaphorin 4B, 4D, and recombinant mouse Semaphorin 4A is observed.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
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Supportive validation
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- Novus Biologicals (provider)
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- Experimental details
- Detection of Sema 4A in Human Blood Monocytes by Flow Cytometry. Human peripheral blood monocytes treated for 24 hours with 50 ng/mL PMA and 500 ng/mL of calcium ionomycin were stained with Mouse Anti-Human Semaphorin 4A Monoclonal Antibody (Catalog # MAB4694, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B).
