Antibody data
- Antibody Data
- Antigen structure
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- Validations
- Flow cytometry [1]
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Validation data
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- Product number
- FAB8166A - Provider product page
- Provider
- Novus Biologicals
- Product name
- Rat Monoclonal Semaphorin 4A Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects mouse Semaphorin 4A in ELISA. Stains HEK293 cells transfected with mouse Semaphorin 4A by Flow Cytometry, but does not stain untransfected cells.
- Reactivity
- Mouse
- Host
- Rat
- Conjugate
- Red dye
- Isotype
- IgG
- Vial size
- 100 Tests
- Storage
- Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 degreesC as supplied.
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Supportive validation
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- Novus Biologicals (provider)
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- Experimental details
- Detection of Semaphorin 4A in HEK293 Human Cell Line Transfected with Human Semaphorin 4A and eGFP by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with mouse Semaphorin 4A and eGFP was stained with either (A) Rat Anti-Mouse Semaphorin 4A APC-conjugated Monoclonal Antibody (Catalog # FAB8166A) or (B) Rat IgG1 Allophycocyanin Isotype Control (Catalog # IC005A). View our protocol for Staining Membrane-associated Proteins.