Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
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Validation data
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- Product number
- MAB7840 - Provider product page
- Provider
- R&D Systems
- Product name
- Human/Mouse Glucokinase/GCK Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects human Glucokinase/GCK in ELISAs. In direct ELISAs, no cross-reactivity with recombinant human PRF-1 is observed.
- Reactivity
- Human, Mouse
- Host
- Mouse
- Conjugate
- Unconjugated
- Antigen sequence
P35557
- Isotype
- IgG
- Antibody clone number
- 849520
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
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Supportive validation
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- R&D Systems (provider)
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- Experimental details
- Detection of Human and Mouse Glucokinase/GCK by Western Blot. Western blot shows lysates of Huh-7 human hepatoma cell line, Hepa 1-6 mouse hepatoma cell line, and HT-2 mouse T cell line. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human Glucokinase/GCK Monoclonal Antibody (Catalog # MAB7840) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Glucokinase/GCK at approximately 52 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.