19666-1-AP

antibody from Proteintech Group

Targeting: GCK HK4, MODY2

Provider product page for 19666-1-AP

Western blot

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Immunohistochemistry

Antibody data

Antibody Data
Antigen structure
References [33]
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Western blot [1]

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Product number: 19666-1-AP - Provider product page
Provider: Proteintech Group
Proper citation: Proteintech Cat#19666-1-AP, RRID:AB_10863656
Product name: GCK antibody
Antibody type: Polyclonal
Description: GCK antibody (Cat. #19666-1-AP) is a rabbit polyclonal antibody that shows reactivity with mouse, rat, human and has been validated for the following applications: IHC, IP, WB,ELISA.
Reactivity: Human, Mouse, Rat
Host: Rabbit
Conjugate: Unconjugated
Isotype: IgG
Vial size: 20ul, 150ul

GCK protein structure - 19666-1-AP shown in red.

Submitted references Glucokinase activity suppresses hepatic cholesterol synthesis and triglyceride accumulation: A new model for the effects of the GKRP P466L common human variant.

Santoleri D, Traynor S, Gavin MJ, Merrick D, Seale P, Titchenell PM
bioRxiv : the preprint server for biology 2026 Apr 8;

GLP-1 receptor agonist protects glucose-stimulated insulin secretion in pancreatic β-cells against lipotoxicity via PPARδ/UCP2 pathway.

Liu Z, Chen Y, Su Y, Peng Y, Xu F, Yao B, Liang H, Lin B, Xu W
Cellular and molecular life sciences : CMLS 2025 Oct 30;82(1):375

Continuous Exposure of Nonobese Adult Male Rats to a Soft-Textured, Readily Absorbable Diet Induces Insulin Resistance and Derangements in Hepatic Glucose and Lipid Metabolism.

Yamaguchi F, Akieda-Asai S, Nakamura E, Uchida H, Yamashita A, Date Y
The Journal of nutrition 2025 May;155(5):1387-1397

Reversing metabolic reprogramming by CPT1 inhibition with etomoxir promotes cardiomyocyte proliferation and heart regeneration via DUSP1 ADP-ribosylation-mediated p38 MAPK phosphorylation.

Tang L, Shi Y, Liao Q, Wang F, Wu H, Ren H, Wang X, Fu W, Shou J, Wang WE, Jose PA, Yang Y, Zeng C
Acta pharmaceutica Sinica. B 2025 Jan;15(1):256-277

NLRP3 inflammasome activation and disruption of IRS-1/PI3K/AKT signaling: Potential mechanisms of arsenic-induced pancreatic beta cells dysfunction in rats.

Liu Y, Wang W, Liang B, Zou Z, Zhang A
Ecotoxicology and environmental safety 2025 Jan 1;289:117504

Nucleus-translocated glucokinase functions as a protein kinase to phosphorylate TAZ and promote tumour growth.

Zhao G, Luo S, Zhao H, Ma Q, Jiang H, Wang L, Liu J, Guo D, Wang R, Xu Q, Lun J, Xie R, Duan Y, Ma L, Qiu W, Fang J, Lu Z
Nature communications 2025 Aug 4;16(1):7156

β-Cell Dedifferentiation in HOMA-βlow and HOMA-βhigh Subjects.

Kang F, Zhang Z, Fu H, Sun J, Zhang J, Wang Q
The Journal of clinical endocrinology and metabolism 2025 Apr 22;110(5):e1430-e1438

Caloric restriction prevents inheritance of polycystic ovary syndrome through oocyte-mediated DNA methylation reprogramming.

Liu Y, Dong Y, Jiang Y, Han S, Liu X, Xu X, Zhu A, Zhao Z, Gao Y, Zou Y, Zhang C, Bian Y, Zhang Y, Liu J, Zhao S, Zhao H, Chen ZJ
Cell metabolism 2025 Apr 1;37(4):920-935.e6

The structures of two polysaccharides from Fructus Corni and their protective effect on insulin resistance.

Ma GY, Hou LK, Guo JH, Sun JC, Nong Q, Wang YF, Hu SW, Zhao WJ, Tan J, Liu XF, Guo Y, Zhang BJ, Wang HL, Wang SY, Li XQ, Cao W
Carbohydrate polymers 2025 Apr 1;353:123290

Gentiopicroside ameliorates glucose and lipid metabolism in T2DM via targeting FGFR1.

Xu Z, Huang J, Wen M, Zhang X, Lyu D, Li S, Xiao H, Li M, Shen C, Huang H
Phytomedicine : international journal of phytotherapy and phytopharmacology 2024 Sep;132:155780

Amino acid is a major carbon source for hepatic lipogenesis.

Liao Y, Chen Q, Liu L, Huang H, Sun J, Bai X, Jin C, Li H, Sun F, Xiao X, Zhang Y, Li J, Han W, Fu S
Cell metabolism 2024 Nov 5;36(11):2437-2448.e8

Insulin receptor isoform B is required for efficient proinsulin processing in pancreatic β cells.

Jiang M, Wang N, Zhang Y, Zhang J, Li Y, Yan X, Zhang H, Li C, Guan Y, Liang B, Zhang W, Wu Y
iScience 2024 Jul 19;27(7):110017

β-Cell glucokinase expression was increased in type 2 diabetes subjects with better glycemic control.

Liu J, Fu H, Kang F, Ning G, Ni Q, Wang W, Wang Q
Journal of diabetes 2023 May;15(5):409-418

Cyb5r3-based mechanism and reversal of secondary failure to sulfonylurea in diabetes.

Watanabe H, Du W, Son J, Sui L, Asahara SI, Kurland IJ, Kuo T, Kitamoto T, Miyachi Y, de Cabo R, Accili D
Science translational medicine 2023 Feb;15(681):eabq4126

Effects of propranolol on glucose metabolism in hemangioma-derived endothelial cells.

Yang K, Li X, Qiu T, Zhou J, Gong X, Lan Y, Ji Y
Biochemical pharmacology 2023 Dec;218:115922

Mof plays distinct roles in hepatic lipid metabolism under healthy or non-alcoholic fatty liver conditions.

Guo X, Liang K, Xia L, Zhang X, Liu J, Wang C, Li J, Li X, Hou X, Chen L
iScience 2023 Dec 15;26(12):108446

Hypoglycemic flavonoids from Selaginella tamariscina (P.Beauv.) Spring.

Long HP, Liu J, Xu PS, Xu KP, Li J, Tan GS
Phytochemistry 2022 Mar;195:113073

GRASP55 regulates the unconventional secretion and aggregation of mutant huntingtin.

Ahat E, Bui S, Zhang J, da Veiga Leprevost F, Sharkey L, Reid W, Nesvizhskii AI, Paulson HL, Wang Y
The Journal of biological chemistry 2022 Aug;298(8):102219

Functionalized gadofullerene ameliorates impaired glycolipid metabolism in type 2 diabetic mice.

Wu J, Chen Y, Li X, Ran L, Liu X, Wang X, Zhen M, Shao S, Zeng L, Wang C, Liang B, Zhao J, Wu Y
Journal of genetics and genomics = Yi chuan xue bao 2022 Apr;49(4):364-376

Citrullination of glucokinase is linked to autoimmune diabetes.

Yang ML, Horstman S, Gee R, Guyer P, Lam TT, Kanyo J, Perdigoto AL, Speake C, Greenbaum CJ, Callebaut A, Overbergh L, Kibbey RG, Herold KC, James EA, Mamula MJ
Nature communications 2022 Apr 6;13(1):1870

FABP3 Deficiency Exacerbates Metabolic Derangement in Cardiac Hypertrophy and Heart Failure via PPARα Pathway.

Zhuang L, Mao Y, Liu Z, Li C, Jin Q, Lu L, Tao R, Yan X, Chen K
Frontiers in cardiovascular medicine 2021;8:722908

Diurnal regulation of oxidative phosphorylation restricts hepatocyte proliferation and inflammation.

Bai X, Liao Y, Sun F, Xiao X, Fu S
Cell reports 2021 Sep 7;36(10):109659

Loss of hepatic aldolase B activates Akt and promotes hepatocellular carcinogenesis by destabilizing the Aldob/Akt/PP2A protein complex.

He X, Li M, Yu H, Liu G, Wang N, Yin C, Tu Q, Narla G, Tao Y, Cheng S, Yin H
PLoS biology 2020 Dec;18(12):e3000803

Therapeutic potential of NaoXinTong Capsule on the developed diabetic nephropathy in db/db mice.

Yang S, Chen Y, Duan Y, Ma C, Liu L, Li Q, Yang J, Li X, Zhao B, Wang Y, Qian K, Liu M, Zhu Y, Yang X, Han J
Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie 2019 Oct;118:109389

Adlay Bran Oil Suppresses Hepatic Gluconeogenesis and Attenuates Hyperlipidemia in Type 2 Diabetes Rats.

Tseng YH, Chang CW, Chiang W, Hsieh SC
Journal of medicinal food 2019 Jan;22(1):22-28

Retinol binding protein 4 mediates MEHP-induced glucometabolic abnormalities in HepG2 cells.

Wang F, Chang C, Li R, Zhang Z, Jiang H, Zeng N, Li D, Chen L, Xiao Y, Chen W, Wang Q
Toxicology 2019 Aug 1;424:152236

Inhibition of hexokinases holds potential as treatment strategy for rheumatoid arthritis.

Song G, Lu Q, Fan H, Zhang X, Ge L, Tian R, Wang S, Feng T, Pan J, Feng J, Xiao Y, Yi X, Ren N, Wang L
Arthritis research & therapy 2019 Apr 3;21(1):87

NaoXinTong Capsules inhibit the development of diabetic nephropathy in db/db mice.

Yang S, Liu M, Chen Y, Ma C, Liu L, Zhao B, Wang Y, Li X, Zhu Y, Gao X, Kong D, Duan Y, Han J, Yang X
Scientific reports 2018 Jun 14;8(1):9158

Glutaredoxins concomitant with optimal ROS activate AMPK through S-glutathionylation to improve glucose metabolism in type 2 diabetes.

Dong K, Wu M, Liu X, Huang Y, Zhang D, Wang Y, Yan LJ, Shi D
Free radical biology & medicine 2016 Dec;101:334-347

ROS-mediated glucose metabolic reprogram induces insulin resistance in type 2 diabetes.

Dong K, Ni H, Wu M, Tang Z, Halim M, Shi D
Biochemical and biophysical research communications 2016 Aug 5;476(4):204-211

Efficacy of Berberine in Patients with Non-Alcoholic Fatty Liver Disease.

Yan HM, Xia MF, Wang Y, Chang XX, Yao XZ, Rao SX, Zeng MS, Tu YF, Feng R, Jia WP, Liu J, Deng W, Jiang JD, Gao X
PloS one 2015;10(8):e0134172

Administration of Danhong Injection to diabetic db/db mice inhibits the development of diabetic retinopathy and nephropathy.

Liu M, Pan Q, Chen Y, Yang X, Zhao B, Jia L, Zhu Y, Zhang B, Gao X, Li X, Han J, Duan Y
Scientific reports 2015 Jun 10;5:11219

Proteomic study reveals a co-occurrence of gallic acid-induced apoptosis and glycolysis in B16F10 melanoma cells.

Liu C, Lin JJ, Yang ZY, Tsai CC, Hsu JL, Wu YJ
Journal of agricultural and food chemistry 2014 Dec 3;62(48):11672-80

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Submitted by: Proteintech Group (provider)
Main image
Experimental details: The GCK antibody from Proteintech is a rabbit polyclonal antibody to a fusion protein of human GCK. This antibody recognizes human, mouse, rat antigen. The GCK antibody has been validated for the following applications: ELISA, WB analysis.
Appendix: 19666-1-AP.pdf

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