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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [2]
- Immunocytochemistry [2]
- Immunohistochemistry [1]
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- Product number
- GTX111517 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX111517, RRID:AB_1950353
- Product name
- Glucokinase antibody [N1C1-2]
- Antibody type
- Polyclonal
- Reactivity
- Human
- Host
- Rabbit
Submitted references Upregulation of Glucose Uptake and Hexokinase Activity of Primary Human CD4+ T Cells in Response to Infection with HIV-1.
Kavanagh Williamson M, Coombes N, Juszczak F, Athanasopoulos M, Khan MB, Eykyn TR, Srenathan U, Taams LS, Dias Zeidler J, Da Poian AT, Huthoff H
Viruses 2018 Mar 7;10(3)
Viruses 2018 Mar 7;10(3)
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Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Sample (30 ?g of whole cell lysate) A: H1299 B: HeLa 10% SDS PAGE GTX111517 diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- GCK antibody detects GCK protein by western blot analysis.A. 30 ?g Huh7 whole cell lysate/extractB. 30 ?g Hep3B whole cell lysate/extractC. 30 ?g HepG2 whole cell lysate/extract10% SDS-PAGEGCK antibody (GTX111517) dilution: 1:1000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of paraformaldehyde-fixed A549, using Glucokinase(GTX111517) antibody at 1:200 dilution.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Glucokinase antibody [N1C1-2] detects Glucokinase protein at cytoplasm by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: Glucokinase protein stained by Glucokinase antibody [N1C1-2] (GTX111517) diluted at 1:500.Blue: Hoechst 33342 staining.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded MCF7 xenograft, using Glucokinase(GTX111517) antibody at 1:500 dilution.
