Explore
Validate
Learn
Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Immunocytochemistry [2]
Submit
Validation data
Reference
Comment
Report error
- Product number
- ABIN2508191 - Provider product page
- Provider
- antibodies-online
- Product name
- anti-Glucokinase (Hexokinase 4) (GCK) (AA 1-30), (N-Term) antibody
- Antibody type
- Polyclonal
- Antigen
- This GCK antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human GCK.Antigen type: Synthetic Peptide
- Description
- This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Epitope
- AA 1-30,N-Term
- Isotype
- IgG
- Vial size
- 200 μL
- Storage
- Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
No comments: Submit comment
Supportive validation
- Submitted by
- antibodies-online (provider)
- Main image
- Experimental details
- ATTO ® dyes can be used for multicolor immunofluorescent detection with low background and high signal. Examples shown are: A. Tubulin in PtK2- male Rat Kangaroo Kidney Epithelial Cells was detected using ATTO 532 labeled secondary antibody. B. Muscle alpha-actin was stained with a mouse primary antibody and ATTO 488 anti-mouse IgG (green) while Cytokeratin was stained with polyclonal rabbit anti-cytokeratin and ATTO 647N anti-rabbit IgG (red). C. HUVEC (Human umbilical vein endothelial cells were stained with anti- Vimentin-ATTO 532 (green), anti-E-Cadherin-ATTO 655 (red) and DAPI (blue). D. Rat colon sections were stained with Anti-Aquaporin 3-ATTO 594 antibody. Hoechst 33342 (blue) is used as counterstain. Images provided courtesy of Dr. Jörg Reichwein, ATTO-TEC GmbH Atto? dyes can be used for multicolor immunofluorescent detection with low background and high signal. Example shown here is Immunohistochemical staining using ATTO-550 Anti-Aquaporin 2-antibody (red) of paraffin embedded region of rat kidney showing a transversal cut of the inner medulla near to the renal papilla. Nuclei are visualized with Hoechst 33342 (blue). Images provided courtesy of Dr. Jörg Reichwein, ATTO-TEC GmbH
- Submitted by
- antibodies-online (provider)
- Main image
- Experimental details
- ATTO ® dyes can be used for multicolor immunofluorescent detection with low background and high signal. Examples shown are: A. Tubulin in PtK2- male Rat Kangaroo Kidney Epithelial Cells was detected using ATTO 532 labeled secondary antibody. B. Muscle alpha-actin was stained with a mouse primary antibody and ATTO 488 anti-mouse IgG (green) while Cytokeratin was stained with polyclonal rabbit anti-cytokeratin and ATTO 647N anti-rabbit IgG (red). C. HUVEC (Human umbilical vein endothelial cells were stained with anti- Vimentin-ATTO 532 (green), anti-E-Cadherin-ATTO 655 (red) and DAPI (blue). D. Rat colon sections were stained with Anti-Aquaporin 3-ATTO 594 antibody. Hoechst 33342 (blue) is used as counterstain. Images provided courtesy of Dr. Jörg Reichwein, ATTO-TEC GmbH Atto? dyes can be used for multicolor immunofluorescent detection with low background and high signal. Example shown here is Immunohistochemical staining using ATTO-550 Anti-Aquaporin 2-antibody (red) of paraffin embedded region of rat kidney showing a transversal cut of the inner medulla near to the renal papilla. Nuclei are visualized with Hoechst 33342 (blue). Images provided courtesy of Dr. Jörg Reichwein, ATTO-TEC GmbH
