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Western blot
ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [3]
- Immunocytochemistry [2]
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- Product number
- PA5-29325 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Anti-GCK Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: 293T, A431, H1299, HeLa, HepG2, Molt-4, Raji, Huh7, Hep3B, HepG2. Predicted reactivity: Mouse (97%), Rat (97%), Zebrafish (86%), Xenopus laevis (87%), Cat (96%), Sheep (97%), Bovine (96%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Glucokinase using A) 30 µg Huh7 whole cell lysate (B) 30 µg Hep3B whole cell lysate and C) 30 µg HepG2 whole cell lysate. Samples were loaded onto a 10% SDS-PAGE gel and probed with a Glucokinase polyclonal antibody (Product # PA5-29325) at a dilution of 1:1000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Glucokinase using 30 µg of A) H1299 and B) HeLa lysate. Samples were loaded onto a 10% SDS-PAGE gel and probed with a Glucokinase polyclonal antibody (Product # PA5-29325) at a dilution of 1:1000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of GCK was performed by separating 30 µg of various whole cell extracts by 10% SDS-PAGE. Proteins were transferred to a membrane and probed with a GCK Polyclonal Antibody (Product # PA5-29325) at a dilution of 1:1000 and a HRP-conjugated anti-rabbit IgG secondary antibody.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of Glucokinase in paraformaldehyde-fixed A549 cells using a Glucokinase polyclonal antibody (Product # PA5-29325) at a 1:200 dilution.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of Glucokinase showing staining in the cytoplasm of HeLa cells. HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min and stained using a Glucokinase polyclonal antibody (Product # PA5-29325) diluted at 1:500. Blue: Hoechst 33342 staining.
